PUBLICATION

Co-translational protein targeting facilitates centrosomal recruitment of PCNT during centrosome maturation in vertebrates

Authors
Sepulveda, G., Antkowiak, M., Brust-Mascher, I., Mahe, K., Ou, T., Castro, N.M., Christensen, L.N., Cheung, L., Jiang, X., Yoon, D., Huang, B., Jao, L.E.
ID
ZDB-PUB-180502-9
Date
2018
Source
eLIFE   7: (Journal)
Registered Authors
Antkowiak, Mark, Jao, Li-En, Sepulveda, Guadalupe, Yoon, Daniel
Keywords
cell biology, human, zebrafish
MeSH Terms
  • Antigens/genetics
  • Antigens/metabolism*
  • Zebrafish Proteins/metabolism
  • Dyneins/metabolism*
  • Cell Cycle Proteins/metabolism
  • Protein Transport
  • Humans
  • Nerve Tissue Proteins/metabolism
  • Animals
  • Protein Biosynthesis*
  • Mitosis*
  • RNA, Messenger/analysis
  • Zebrafish
  • Centrosome/metabolism*
  • Cell Line
(all 15)
PubMed
29708497 Full text @ Elife
Abstract
As microtubule-organizing centers of animal cells, centrosomes guide the formation of the bipolar spindle that segregates chromosomes during mitosis. At mitosis onset, centrosomes maximize microtubule-organizing activity by rapidly expanding the pericentriolar material (PCM). This process is in part driven by the large PCM protein pericentrin (PCNT), as its level increases at the PCM and helps recruit additional PCM components. However, the mechanism underlying the timely centrosomal enrichment of PCNT remains unclear. Here, we show that PCNT is delivered co-translationally to centrosomes during early mitosis by cytoplasmic dynein, as evidenced by centrosomal enrichment of PCNT mRNA, its translation near centrosomes, and requirement of intact polysomes for PCNT mRNA localization. Additionally, the microtubule minus-end regulator, ASPM, is also targeted co-translationally to mitotic spindle poles. Together, these findings suggest that co-translational targeting of cytoplasmic proteins to specific subcellular destinations may be a generalized protein targeting mechanism.
Genes / Markers
Figures
Figure Gallery (3 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
tup2
    Insertion
    tup5
      Small Deletion
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      pcntCRISPR1-pcntCRISPR
      1 - 1 of 1
      Show
      Fish
      1 - 3 of 3
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      Antibodies
      Orthology
      No data available
      Engineered Foreign Genes
      No data available
      Mapping
      No data available