PUBLICATION
Feedback between tissue packing and neurogenesis in the zebrafish neural tube
- Authors
- Hiscock, T.W., Miesfeld, J.B., Mosaliganti, K.R., Link, B.A., Megason, S.G.
- ID
- ZDB-PUB-180422-5
- Date
- 2018
- Source
- Development (Cambridge, England) 145(9): (Journal)
- Registered Authors
- Link, Brian, Megason, Sean, Miesfeld, Joel B., Mosaliganti, Kishore
- Keywords
- Differentiation, Feedback control, Neurogenesis, Tissue packing
- MeSH Terms
-
- Animals
- Cell Differentiation/physiology*
- Cell Proliferation/physiology*
- Neural Stem Cells/cytology
- Neural Stem Cells/metabolism*
- Neural Tube/cytology
- Neural Tube/embryology*
- Neurogenesis/physiology*
- Receptors, Notch/genetics
- Receptors, Notch/metabolism
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 29678815 Full text @ Development
Citation
Hiscock, T.W., Miesfeld, J.B., Mosaliganti, K.R., Link, B.A., Megason, S.G. (2018) Feedback between tissue packing and neurogenesis in the zebrafish neural tube. Development (Cambridge, England). 145(9).
Abstract
Balancing the rate of differentiation and proliferation in developing tissues is essential to produce organs of robust size and composition. Whilst many molecular regulators have been established, how these connect to physical and geometrical aspects of tissue architecture is poorly understood. Here, using high-resolution timelapse imaging, we find that changes to cell geometry associated with dense tissue packing play a significant role in regulating differentiation rate in the zebrafish neural tube. Specifically, progenitors that are displaced away from the apical surface due to crowding tend to differentiate, in a Notch-dependent manner. Using simulations we show that interplay between progenitor density, cell shape, and changes in differentiation rate could naturally result in negative feedback control on progenitor cell number. Given these results, we suggest a model whereby differentiation rate is regulated by density dependent effects on cell geometry to: 1) correct variability in cell number, and 2) balance the rates of proliferation and differentiation over development to "fill" the available space.
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Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
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