PUBLICATION

Synaptically silent sensory hair cells in zebrafish are recruited after damage

Authors
Zhang, Q., Li, S., Wong, H.C., He, X.J., Beirl, A., Petralia, R.S., Wang, Y.X., Kindt, K.S.
ID
ZDB-PUB-180418-24
Date
2018
Source
Nature communications   9: 1388 (Journal)
Registered Authors
Beirl, Alisha, Kindt, Katie, Li, Suna, Wong, Hiu-Tung Candy, Zhang, Qiuxiang
Keywords
none
MeSH Terms
  • Calcium/metabolism
  • Larva/cytology
  • Larva/metabolism
  • Animals
  • Synapses/metabolism*
  • Zebrafish
  • Lateral Line System/growth & development
  • Lateral Line System/injuries
  • Lateral Line System/metabolism
  • Mechanotransduction, Cellular/physiology*
  • Potassium/metabolism
  • Ion Transport
  • Synaptic Transmission/physiology*
  • Embryo, Nonmammalian
  • Regeneration/physiology
  • Hair Cells, Auditory/cytology*
  • Hair Cells, Auditory/metabolism
  • Cations, Divalent
  • Calcium Channels, L-Type/genetics
  • Calcium Channels, L-Type/metabolism*
(all 20)
PubMed
29643351 Full text @ Nat. Commun.
Abstract
Analysis of mechanotransduction among ensembles of sensory hair cells in vivo is challenging in many species. To overcome this challenge, we used optical indicators to investigate mechanotransduction among collections of hair cells in intact zebrafish. Our imaging reveals a previously undiscovered disconnect between hair-cell mechanosensation and synaptic transmission. We show that saturating mechanical stimuli able to open mechanically gated channels are unexpectedly insufficient to evoke vesicle fusion in the majority of hair cells. Although synaptically silent, latent hair cells can be rapidly recruited after damage, demonstrating that they are synaptically competent. Therefore synaptically silent hair cells may be an important reserve that acts to maintain sensory function. Our results demonstrate a previously unidentified level of complexity in sculpting sensory transmission from the periphery.
Genes / Markers
Figures
Figure Gallery (14 images) / 2
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
idc1TgTransgenic Insertion
    idc3TgTransgenic Insertion
      idc6TgTransgenic Insertion
        idc7TgTransgenic Insertion
          idc8TgTransgenic Insertion
            idc9TgTransgenic Insertion
              nl1TgTransgenic Insertion
                vo10TgTransgenic Insertion
                  1 - 8 of 8
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                  Human Disease / Model
                  No data available
                  Sequence Targeting Reagents
                  Target Reagent Reagent Type
                  neurog1MO9-neurog1MRPHLNO
                  1 - 1 of 1
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                  Fish
                  No data available
                  Antibodies
                  Orthology
                  No data available
                  Engineered Foreign Genes
                  Marker Marker Type Name
                  BongwooriEFGBongwoori
                  EGFPEFGEGFP
                  GCaMPEFGGCaMP
                  mCherryEFGmCherry
                  RCaMPEFGRCaMP
                  RGECOEFGRGECO
                  SYPHYEFGSYPHY
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                  Mapping
                  No data available
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                  Citation
                  Zhang, Q., Li, S., Wong, H.C., He, X.J., Beirl, A., Petralia, R.S., Wang, Y.X., Kindt, K.S. (2018) Synaptically silent sensory hair cells in zebrafish are recruited after damage. Nature communications. 9:1388.