PUBLICATION
Bromodomain and Extraterminal (BET) Proteins Regulate Hepatocyte Proliferation in Hepatocyte-Driven Liver Regeneration
- Authors
- Russell, J.O., Ko, S., Saggi, H.S., Singh, S., Poddar, M., Shin, D., Monga, S.P.
- ID
- ZDB-PUB-180317-4
- Date
- 2018
- Source
- The American journal of pathology 188(6): 1389-1405 (Journal)
- Registered Authors
- Ko, Sungjin, Shin, Donghun
- Keywords
- none
- MeSH Terms
-
- Acetaminophen/toxicity
- Analgesics, Non-Narcotic/toxicity
- Animals
- Azepines/pharmacology*
- Cell Proliferation*
- Chemical and Drug Induced Liver Injury/drug therapy
- Chemical and Drug Induced Liver Injury/etiology
- Chemical and Drug Induced Liver Injury/metabolism
- Chemical and Drug Induced Liver Injury/pathology*
- Hep G2 Cells
- Hepatectomy/adverse effects
- Hepatocytes/cytology*
- Hepatocytes/drug effects
- Hepatocytes/physiology
- Humans
- Liver Regeneration*
- Male
- Mice
- Mice, Inbred C57BL
- Proteins/antagonists & inhibitors*
- Signal Transduction
- Triazoles/pharmacology*
- Zebrafish
- PubMed
- 29545201 Full text @ Am. J. Pathol.
Citation
Russell, J.O., Ko, S., Saggi, H.S., Singh, S., Poddar, M., Shin, D., Monga, S.P. (2018) Bromodomain and Extraterminal (BET) Proteins Regulate Hepatocyte Proliferation in Hepatocyte-Driven Liver Regeneration. The American journal of pathology. 188(6):1389-1405.
Abstract
Bromodomain and extraterminal (BET) proteins recruit key components of basic transcriptional machinery to promote gene expression. Aberrant expression and mutations in BET genes have been identified in many malignancies. Small molecule inhibitors of BET proteins like JQ1 have shown efficacy in preclinical cancer models including affecting growth of hepatocellular carcinoma. BET proteins also regulate cell proliferation in nontumor settings. We recently showed that BET proteins regulate cholangiocyte-driven liver regeneration. Here, we studied the role of BET proteins in hepatocyte-driven liver regeneration in partial hepatectomy (PHx) and acetaminophen-induced liver injury models in mice and zebrafish. JQ1 was injected 2 or 16 hours post-PHx in mice to determine effect on hepatic injury, regeneration and signaling. Mice treated with JQ1 after PHx displayed increased liver injury and a near-complete inhibition of hepatocyte proliferation. Levels of Ccnd1 mRNA and Cyclin D1 protein were reduced in 16-hour post-PHx JQ1-injected animals, and even further reduced in 2 hours post-PHx JQ1-injected mice. JQ1-treated zebrafish larvae after acetaminophen-induced injury also displayed notably impaired hepatocyte proliferation. In both models, Wnt signaling was prominently suppressed by JQ1. Our results show that BET proteins regulate hepatocyte proliferation-driven liver regeneration, and Wnt signaling is particularly sensitive to BET protein inhibition.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping