PUBLICATION
Transmission Electron Microscopy for Zebrafish Larvae and Adult Lateral Line Nerve
- Authors
- Cunningham, R.L., Monk, K.R.
- ID
- ZDB-PUB-180317-10
- Date
- 2018
- Source
- Methods in molecular biology (Clifton, N.J.) 1739: 385-400 (Chapter)
- Registered Authors
- Monk, Kelly
- Keywords
- Adult zebrafish lateral line nerve, Lateral line nerve, Motor nerves, Myelin ultrastructure, Transmission electron microscopy
- MeSH Terms
-
- Animals
- Larva/cytology
- Larva/ultrastructure
- Microscopy, Electron, Transmission/methods*
- Myelin Sheath/metabolism
- Schwann Cells/cytology
- Schwann Cells/ultrastructure
- Zebrafish
- PubMed
- 29546722 Full text @ Meth. Mol. Biol.
Citation
Cunningham, R.L., Monk, K.R. (2018) Transmission Electron Microscopy for Zebrafish Larvae and Adult Lateral Line Nerve. Methods in molecular biology (Clifton, N.J.). 1739:385-400.
Abstract
Transmission electron microscopy (TEM) enables visualization of the ultrastructure of the myelin sheath. Schwann cells on the posterior lateral line nerves and motor nerves can be imaged by TEM. Here, we detail the multiday processing of larval trunks and dissected posterior lateral line for TEM, as well as how to trim embedded samples, section, and stain grids for imaging.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping