PUBLICATION

Characterization of Pax3 and Sox10 transgenic Xenopus laevis embryos as tools to study neural crest development

Authors
Alkobtawi, M., Ray, H., Barriga, E.H., Moreno, M., Kerney, R., Monsoro-Burq, A.H., Saint-Jeannet, J.P., Mayor, R.
ID
ZDB-PUB-180310-12
Date
2018
Source
Developmental Biology   444 Suppl 1: S202-S208 (Journal)
Registered Authors
Mayor, Roberto
Keywords
Neural crest, Pax3, Sox10, induction, migration, transgenic
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Cell Differentiation
  • Gene Expression Regulation, Developmental/genetics
  • Genetic Engineering/methods
  • Green Fluorescent Proteins
  • Humans
  • Neural Crest/embryology
  • Neural Crest/metabolism*
  • Neural Crest/physiology
  • Neurogenesis
  • PAX3 Transcription Factor/metabolism*
  • PAX3 Transcription Factor/physiology
  • SOXE Transcription Factors/metabolism*
  • SOXE Transcription Factors/physiology
  • Xenopus laevis/embryology
PubMed
29522707 Full text @ Dev. Biol.
Abstract
The neural crest is a multipotent population of cells that originates a variety of cell types. Many animal models are used to study neural crest induction, migration and differentiation, with amphibians and birds being the most widely used systems. A major technological advance to study neural crest development in mouse, chick and zebrafish has been the generation of transgenic animals in which neural crest specific enhancers/promoters drive the expression of either fluorescent proteins for use as lineage tracers, or modified genes for use in functional studies. Unfortunately, no such transgenic animals currently exist for the amphibians Xenopus laevis and tropicalis, key model systems for studying neural crest development. Here we describe the generation and characterization of two transgenic Xenopus laevis lines, Pax3-GFP and Sox10-GFP, in which GFP is expressed in the pre-migratory and migratory neural crest, respectively. We show that Pax3-GFP could be a powerful tool to study neural crest induction, whereas Sox10-GFP could be used in the study of neural crest migration in living embryos.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping