PUBLICATION
CEP128 Localizes to the Subdistal Appendages of the Mother Centriole and Regulates TGF-β/BMP Signaling at the Primary Cilium
- Authors
- Mönnich, M., Borgeskov, L., Breslin, L., Jakobsen, L., Rogowski, M., Doganli, C., Schrøder, J.M., Mogensen, J.B., Blinkenkjær, L., Harder, L.M., Lundberg, E., Geimer, S., Christensen, S.T., Andersen, J.S., Larsen, L.A., Pedersen, L.B.
- ID
- ZDB-PUB-180308-6
- Date
- 2018
- Source
- Cell Reports 22: 2584-2592 (Journal)
- Registered Authors
- Doganli, Canan, Monnich, Maren
- Keywords
- BMP, CEP128, TGF-β, basal body, bone morphogenetic protein, centriole, centrosome, phosphoproteomics, primary cilium, subdistal appendage, transforming growth factor β, zebrafish
- MeSH Terms
-
- Animals
- Bone Morphogenetic Proteins/metabolism*
- Centrioles/metabolism*
- Centrosome/metabolism
- Cilia/metabolism*
- Humans
- Microtubule Proteins/metabolism*
- Protein Transport
- Transforming Growth Factor beta/metabolism*
- Zebrafish/metabolism*
- Zebrafish Proteins/metabolism*
- rab GTP-Binding Proteins/metabolism
- PubMed
- 29514088 Full text @ Cell Rep.
Citation
Mönnich, M., Borgeskov, L., Breslin, L., Jakobsen, L., Rogowski, M., Doganli, C., Schrøder, J.M., Mogensen, J.B., Blinkenkjær, L., Harder, L.M., Lundberg, E., Geimer, S., Christensen, S.T., Andersen, J.S., Larsen, L.A., Pedersen, L.B. (2018) CEP128 Localizes to the Subdistal Appendages of the Mother Centriole and Regulates TGF-β/BMP Signaling at the Primary Cilium. Cell Reports. 22:2584-2592.
Abstract
The centrosome is the main microtubule-organizing center in animal cells and comprises a mother and daughter centriole surrounded by pericentriolar material. During formation of primary cilia, the mother centriole transforms into a basal body that templates the ciliary axoneme. Ciliogenesis depends on mother centriole-specific distal appendages, whereas the role of subdistal appendages in ciliary function is unclear. Here, we identify CEP128 as a centriole subdistal appendage protein required for regulating ciliary signaling. Loss of CEP128 did not grossly affect centrosomal or ciliary structure but caused impaired transforming growth factor-β/bone morphogenetic protein (TGF-β/BMP) signaling in zebrafish and at the primary cilium in cultured mammalian cells. This phenotype is likely the result of defective vesicle trafficking at the cilium as ciliary localization of RAB11 was impaired upon loss of CEP128, and quantitative phosphoproteomics revealed that CEP128 loss affects TGF-β1-induced phosphorylation of multiple proteins that regulate cilium-associated vesicle trafficking.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping