PUBLICATION
MicroRNA-27b Depletion Enhances Endotrophic and Intravascular Lipid Accumulation and Induces Adipocyte Hyperplasia in Zebrafish
- Authors
- Hsu, C.C., Lai, C.Y., Lin, C.Y., Yeh, K.Y., Her, G.M.
- ID
- ZDB-PUB-171230-6
- Date
- 2017
- Source
- International Journal of Molecular Sciences 19(1): (Journal)
- Registered Authors
- Her, Guor Muor
- Keywords
- adipogenesis, lipogenesis, liver steatosis, microRNA sponge, nonalcoholic fatty liver disease
- MeSH Terms
-
- Adipocytes/metabolism
- Adipocytes/pathology
- Adipogenesis/genetics*
- Animals
- Animals, Genetically Modified
- Antagomirs/genetics
- Antagomirs/metabolism
- Base Sequence
- CCAAT-Enhancer-Binding Proteins/genetics
- CCAAT-Enhancer-Binding Proteins/metabolism
- Disease Models, Animal
- Embryo, Nonmammalian
- Gene Expression Regulation, Developmental
- Genes, Reporter
- Green Fluorescent Proteins/genetics
- Green Fluorescent Proteins/metabolism
- Hyperlipidemias/genetics*
- Hyperlipidemias/metabolism
- Hyperlipidemias/pathology
- Hyperplasia/genetics*
- Hyperplasia/metabolism
- Hyperplasia/pathology
- Larva/genetics*
- Larva/growth & development
- Larva/metabolism
- Lipid Metabolism/genetics
- Luminescent Proteins/genetics
- Luminescent Proteins/metabolism
- MicroRNAs/antagonists & inhibitors
- MicroRNAs/genetics*
- MicroRNAs/metabolism
- Non-alcoholic Fatty Liver Disease/genetics*
- Non-alcoholic Fatty Liver Disease/metabolism
- Non-alcoholic Fatty Liver Disease/pathology
- PPAR gamma/genetics
- PPAR gamma/metabolism
- Signal Transduction
- Sterol Regulatory Element Binding Protein 1/genetics
- Sterol Regulatory Element Binding Protein 1/metabolism
- Zebrafish
- PubMed
- 29286302 Full text @ Int. J. Mol. Sci.
Citation
Hsu, C.C., Lai, C.Y., Lin, C.Y., Yeh, K.Y., Her, G.M. (2017) MicroRNA-27b Depletion Enhances Endotrophic and Intravascular Lipid Accumulation and Induces Adipocyte Hyperplasia in Zebrafish. International Journal of Molecular Sciences. 19(1).
Abstract
miR-27b has emerged as a regulatory hub in cholesterol and lipid metabolism, and as a potential therapeutic target for treating atherosclerosis and obesity. However, the impact of miR-27b on lipid levels in vivo remains to be determined. Zebrafish lipids are normally stored as triacylglycerols (TGs) and their main storage sites are visceral, intramuscular, and subcutaneous lipid depots, and not blood vessels and liver. In this study, we applied microRNA-sponge (miR-SP) technology and generated zebrafish expressing transgenic miR-27b-SP (C27bSPs), which disrupted endogenous miR-27b activity and induced intravascular lipid accumulation (hyperlipidemia) and the early onset of nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH). Oil Red O staining predominantly increased in the blood vessels and livers of larvae and juvenile C27bSPs, indicating that miR-27b depletion functionally promoted lipid accumulation. C27bSPs also showed an increased weight gain with larger fat pads, resulting from adipocyte hyperplasia. Molecular analysis revealed that miR-27b depletion increased the expression of genes that are associated with lipogenesis and the endoplasmic reticulum (ER). Moreover, miR-27b-SP increased peroxisome proliferator-activated receptor γ (PPAR-γ), CCAAT enhancer binding protein-α (C/EBP-α, and sterol regulatory element binding transcription factor 1c (SREBP-1c) expression and contributed to lipogenesis and adipogenesis.
Conclusion Our results suggest that miR-27b-SP acts as a lipid enhancer by directly increasing the expression of several lipogenic/adipogenic transcriptional factors, resulting in increased lipogenesis and adipogenesis. In this study, miR-27b expression improved lipid metabolism in C27bSPs, which suggests that miR-27b is an important lipogenic factor in regulating early onset of hyperlipidemia and adipogenesis in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping