PUBLICATION
MicroRNA-133b Negatively Regulates Zebrafish Single Mauthner-Cell Axon Regeneration through Targeting tppp3 in Vivo.
- Authors
- Huang, R., Chen, M., Yang, L., Wagle, M., Guo, S., Hu, B.
- ID
- ZDB-PUB-171207-5
- Date
- 2017
- Source
- Frontiers in molecular neuroscience 10: 375 (Journal)
- Registered Authors
- Guo, Su, Hu, Bing, Wagle, Mahendra
- Keywords
- axon regeneration, in vivo imaging, miR-133b, single-cell electroporation, single-cell level, tppp3
- MeSH Terms
- none
- PubMed
- 29209165 Full text @ Front. Mol. Neurosci.
Citation
Huang, R., Chen, M., Yang, L., Wagle, M., Guo, S., Hu, B. (2017) MicroRNA-133b Negatively Regulates Zebrafish Single Mauthner-Cell Axon Regeneration through Targeting tppp3 in Vivo.. Frontiers in molecular neuroscience. 10:375.
Abstract
Axon regeneration, fundamental to nerve repair, and functional recovery, relies on rapid changes in gene expression attributable to microRNA (miRNA) regulation. MiR-133b has been proved to play an important role in different organ regeneration in zebrafish, but its role in regulating axon regeneration in vivo is still controversial. Here, combining single-cell electroporation with a vector-based miRNA-expression system, we have modulated the expression of miR-133b in Mauthner-cells (M-cells) at the single-cell level in zebrafish. Through in vivo imaging, we show that overexpression of miR-133b inhibits axon regeneration, whereas down-regulation of miR-133b, promotes axon outgrowth. We further show that miR-133b regulates axon regeneration by directly targeting a novel regeneration-associated gene, tppp3, which belongs to Tubulin polymerization-promoting protein family. Gain or loss-of-function of tppp3 experiments indicated that tppp3 was a novel gene that could promote axon regeneration. In addition, we observed a reduction of mitochondrial motility, which have been identified to have a positive correlation with axon regeneration, in miR-133b overexpressed M-cells. Taken together, our work provides a novel way to study the role of miRNAs in individual cell and establishes a critical cell autonomous role of miR-133b in zebrafish M-cell axon regeneration. We propose that up-regulation of the newly founded regeneration-associated gene tppp3 may enhance axonal regeneration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping