PUBLICATION

Gdf3 is required for robust Nodal signaling during germ layer formation and left-right patterning

Authors

Pelliccia, J.L., Jindal, G.A., Burdine, R.D.

ID

ZDB-PUB-171117-7

Date

2017

Source

eLIFE 6: (Journal)

Registered Authors

Burdine, Rebecca

Keywords

developmental biology, stem cells, zebrafish

MeSH Terms

Zebrafish Proteins/metabolism*
Germ Layers/embryology*
Zebrafish/embryology*
Nodal Protein/metabolism*
Transforming Growth Factor beta/metabolism*
Signal Transduction*
Animals
Body Patterning*

(all 8)

PubMed

29140250 Full text @ Elife

Abstract

Vertebrate embryonic patterning depends on signaling from Nodal, a TGFβ superfamily member. There are three Nodal orthologs in zebrafish; southpaw directs left-right asymmetries, while squint and cyclops function earlier to pattern mesendoderm. TGFβ member Vg1 is implicated in mesoderm formation but the role of the zebrafish ortholog, Growth differentiation factor 3 (Gdf3), has not been fully explored. We show that zygotic expression of gdf3 is dispensable for embryonic development, while maternally deposited gdf3 is required for mesendoderm formation and dorsal-ventral patterning. We further show that Gdf3 can affect left-right patterning at multiple stages, including proper development of regional cell morphology in Kupffer's vesicle and the establishment of southpaw expression in the lateral plate mesoderm. Collectively, our data indicate that gdf3 is critical for robust Nodal signaling at multiple stages in zebrafish embryonic development.

Genes / Markers

Figures

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Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT + MO1-ndr2 + MO4-ndr1	standard conditions	Prim-5	caudal fin morphology, abnormal	Fig. 3
WT + MO1-ndr2 + MO4-ndr1	standard conditions	Prim-5	endoderm formation decreased occurrence, abnormal	Fig. 3
WT + MO1-ndr2 + MO4-ndr1	standard conditions	Prim-5	mesoderm formation decreased occurrence, abnormal	Fig. 3
WT + MO1-tbxta	control	10-13 somites	lateral plate mesoderm right side spaw expression mislocalised, abnormal	Fig. 4
WT + MO1-tbxta + MO2-gdf3	control	10-13 somites	lateral plate mesoderm spaw expression absent, abnormal	Fig. 4
WT + MO2-gdf3	control	10-13 somites	Kupffer's vesicle dand5 expression spatial pattern, abnormal	Fig. 4
WT + MO2-gdf3	control	10-13 somites	Kupffer's vesicle left side dand5 expression mislocalised, abnormal	Fig. 4
WT + MO2-gdf3	control	10-13 somites	lateral plate mesoderm spaw expression absent, abnormal	Fig. 4
gdf3^pr05/+	standard conditions	30%-epiboly	margin lft1 expression absent, abnormal	Fig. 2
gdf3^pr05/+	standard conditions	30%-epiboly	margin ndr2 expression absent, abnormal	Fig. 2

1 - 10 of 37

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
pr05		Small Deletion	gdf3
pr06		Indel	gdf3
pr11		Indel	gdf3
s870Tg	Tg(sox17:GFP)	Transgenic Insertion

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
gdf3	CRISPR1-gdf3	CRISPR
gdf3	CRISPR2-gdf3	CRISPR
gdf3	MO2-gdf3	MRPHLNO
ndr1	MO4-ndr1	MRPHLNO
ndr2	MO1-ndr2	MRPHLNO
tbxta	MO1-tbxta	MRPHLNO

Fish

Fish
gdf3^pr05/pr05
gdf3^pr05/pr05
gdf3^pr05/pr05 + MO1-ndr2 + MO4-ndr1
gdf3^pr05/+
gdf3^pr05/+
gdf3^pr05
s870Tg
s870Tg + MO2-gdf3
WT
WT + MO1-ndr2 + MO4-ndr1

Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
GFP	EFG	GFP

Mapping

Pelliccia et al., 2017 ZDB-PUB-171117-7 PMID:29140250

Gdf3 is required for robust Nodal signaling during germ layer formation and left-right patterning

Pelliccia et al., 2017

ZDB-PUB-171117-7
PMID:29140250