PUBLICATION
Genetic and neuronal regulation of sleep by neuropeptide VF
- Authors
- Lee, D.A., Andreev, A., Truong, T.V., Chen, A., Hill, A.J., Oikonomou, G., Pham, U., Hong, Y.K., Tran, S., Glass, L., Sapin, V., Engle, J., Fraser, S.E., Prober, D.A.
- ID
- ZDB-PUB-171107-11
- Date
- 2017
- Source
- eLIFE 6: (Journal)
- Registered Authors
- Fraser, Scott E., Prober, David
- Keywords
- neuroscience, zebrafish
- MeSH Terms
-
- Zebrafish
- Animals
- Neurons/physiology
- Signal Transduction
- Sleep*
- Neuropeptides/metabolism*
- Gene Expression Regulation*
- PubMed
- 29106375 Full text @ Elife
Citation
Lee, D.A., Andreev, A., Truong, T.V., Chen, A., Hill, A.J., Oikonomou, G., Pham, U., Hong, Y.K., Tran, S., Glass, L., Sapin, V., Engle, J., Fraser, S.E., Prober, D.A. (2017) Genetic and neuronal regulation of sleep by neuropeptide VF. eLIFE. 6.
Abstract
Sleep is an essential and phylogenetically conserved behavioral state, but it remains unclear to what extent genes identified in invertebrates also regulate vertebrate sleep. RFamide-related neuropeptides have been shown to promote invertebrate sleep, and here we report that the vertebrate hypothalamic RFamide neuropeptide VF (NPVF) regulates sleep in the zebrafish, a diurnal vertebrate. We found that NPVF signaling and npvf-expressing neurons are both necessary and sufficient to promote sleep, that mature peptides derived from the NPVF preproprotein promote sleep in a synergistic manner, and that stimulation of npvf-expressing neurons induces neuronal activity levels consistent with normal sleep. These results identify NPVF signaling and npvf-expressing neurons as a novel vertebrate sleep-promoting system and suggest that RFamide neuropeptides participate in an ancient and central aspect of sleep control.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping