PUBLICATION
Effects of diisononyl phthalate on Danio rerio reproduction
- Authors
- Santangeli, S., Maradonna, F., Zanardini, M., Notarstefano, V., Gioacchini, G., Forner-Piquer, I., Habibi, H., Carnevali, O.
- ID
- ZDB-PUB-170917-8
- Date
- 2017
- Source
- Environmental pollution (Barking, Essex : 1987) 231: 1051-1062 (Journal)
- Registered Authors
- Carnevali, Oliana
- Keywords
- Endocrine disruption, Female fertility, Phthalates, Reproduction
- MeSH Terms
-
- Animals
- Diethylhexyl Phthalate
- Female
- Phthalic Acids/toxicity*
- Plasticizers/analysis
- Polyvinyl Chloride
- Reproduction/drug effects
- Toxicity Tests*
- Water Pollutants, Chemical/toxicity*
- Zebrafish/physiology*
- PubMed
- 28915543 Full text @ Environ. Pollut.
Citation
Santangeli, S., Maradonna, F., Zanardini, M., Notarstefano, V., Gioacchini, G., Forner-Piquer, I., Habibi, H., Carnevali, O. (2017) Effects of diisononyl phthalate on Danio rerio reproduction. Environmental pollution (Barking, Essex : 1987). 231:1051-1062.
Abstract
Di-isononyl phthalate (DiNP) is a high molecular weight phthalate commonly used as a plasticizer. It was introduced as a replacement for bis (2-ethylhexyl) phthalate (DEHP) which is used in the production of plasticized polyvinyl chloride (PVC). The purpose of this study was to investigate for the first time the effect of DiNP on female reproductive physiology in Danio rerio. Fish were exposed to five different doses of DiNP plus control (0 μg/L; 0.42 μg/L; 4.2 μg/L; 42 μg/L; 420 μg/L; 4200 μg/L) for a period of 21 days. We evaluated fish fecundity, oocyte growth, autophagic and apoptotic processes, as well as changes in morphological and biochemical composition of oocytes, using, qPCR analysis, histology and Fourier transform infrared imaging. The results demonstrate a non-monotonic dose response to DiNP. Greater differences were observed at the lowest (0.42 μg/L) and higher concentrations (420 μg/L; 4200 μg/L) of DiNP. The findings provide evidence that exposure to DiNP adversely affect oocytes growth and maturation, leading to abnormal gonadal development and reproduction in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping