PUBLICATION
Neutralization of viral infectivity by zebrafish c-reactive protein isoforms
- Authors
- Bello-Perez, M., Falco, A., Medina-Gali, R., Pereiro, P., Encinar, J.A., Novoa, B., Perez, L., Coll, J.
- ID
- ZDB-PUB-170916-1
- Date
- 2017
- Source
- Molecular immunology 91: 145-155 (Journal)
- Registered Authors
- Novoa, Beatriz
- Keywords
- CRP, SVCV, VHSV, anti-viral neutralizing activity, c-reactive protein, microarrays, zebrafish
- MeSH Terms
-
- Animals
- C-Reactive Protein*/genetics
- C-Reactive Protein*/immunology
- Fish Diseases*/genetics
- Fish Diseases*/immunology
- Fish Diseases*/virology
- Homeodomain Proteins/genetics
- Homeodomain Proteins/immunology
- Mutation
- Protein Isoforms/genetics
- Protein Isoforms/immunology
- Rhabdoviridae/immunology*
- Zebrafish*/genetics
- Zebrafish*/immunology
- Zebrafish*/virology
- PubMed
- 28915434 Full text @ Mol. Immunol.
Citation
Bello-Perez, M., Falco, A., Medina-Gali, R., Pereiro, P., Encinar, J.A., Novoa, B., Perez, L., Coll, J. (2017) Neutralization of viral infectivity by zebrafish c-reactive protein isoforms. Molecular immunology. 91:145-155.
Abstract
This work explores the unexpected in vivo and in vitro anti-viral functions of the seven c-reactive protein (crp1-7) genes of zebrafish (Danio rerio). First results showed heterogeneous crp1-7 transcript levels in healthy wild-type zebrafish tissues and organs and how those levels heterogeneously changed not only after bacterial but also after viral infections, including those in adaptive immunity-deficient rag1-/- mutants. As shown by microarray hybridization and proteomic techniques, crp2/CRP2 and crp5/CRP5 transcripts/proteins were among the most modulated during in vivo viral infection situations including the highest responses in the absence of adaptive immunity. In contrast crp1/CRP1/and crp7/CRP7 very often remained unmodulated. All evidences suggested that zebrafish crp2-6/CRP2-6 may have in vivo anti-viral activities in addition to their well known anti-bacterial and/or physiological functions in mammalians. Confirming those expectations, in vitro neutralization and in vivo protection against spring viremia carp virus (SVCV) infections were demonstrated by crp2-6/CRP2-6 using crp1-7 transfected and/or CRP1-7-enriched supernatant-treated fish cells and crp2-5-injected one-cell stage embryo eggs, respectively. All these findings discovered a crp1-7/CRP1-7 primitive anti-viral functional diversity.These findings may help to study similar functions on the one-gene-coded human CRP, which is widely used as a clinical biomarker for bacterial infections, tissue inflammation and coronary heart diseases.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping