PUBLICATION
Quantitative Responses of Adult Zebrafish to Changes in Ambient Illumination
- Authors
- Shao, E., Bai, Q., Zhou, Y., Burton, E.A.
- ID
- ZDB-PUB-170915-4
- Date
- 2017
- Source
- Zebrafish 14(6): 508-516 (Journal)
- Registered Authors
- Burton, Edward A.
- Keywords
- adult zebrafish, aging, automated behavioral assays, light transition response
- MeSH Terms
-
- Animals
- Behavior, Animal/radiation effects
- Light
- Locomotion/radiation effects
- Motor Activity/physiology*
- Motor Activity/radiation effects
- Photic Stimulation/methods*
- Swimming/physiology*
- Zebrafish/physiology*
- PubMed
- 28910236 Full text @ Zebrafish
Citation
Shao, E., Bai, Q., Zhou, Y., Burton, E.A. (2017) Quantitative Responses of Adult Zebrafish to Changes in Ambient Illumination. Zebrafish. 14(6):508-516.
Abstract
The use of zebrafish models to study central nervous system aging and late-onset neurological diseases will be facilitated by assays allowing rapid evaluation of neurological phenotypes in adult zebrafish. We analyzed groups of 12 adult zebrafish swimming simultaneously in single-animal arenas, and quantified their responses to changes in ambient illumination. Under these conditions, stereotypical locomotor patterns were observed and readily quantified using open source software. Continuous, low-velocity movements were observed during 10-min periods of darkness, whereas intermittent high-velocity movements occurred in bright light. At 80%-90% of abrupt light-to-dark or dark-to-light transitions, adult zebrafish produced a synchronous short-latency (20-22 ms) turn, followed by a propulsive movement with a high transient maximum velocity (400-500 mm/s). Between 5 and 35 months of age, latency increased by ∼10%, and peak velocity decreased by ∼30%, suggesting that the response declines in aged adults. Light transition responses can be measured rapidly and automatically in multiple adult zebrafish simultaneously, providing a convenient quantitative method for evaluating sensorimotor function in adult zebrafish models of neurological disease.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping