PUBLICATION

Promoter architecture and transcriptional regulation of musculoskeletal embryonic nuclear protein 1b (mustn1b) gene in zebrafish

Authors
Suarez-Bregua, P., Chien, C.J., Megias, M., Du, S.D., Rotllant, J.
ID
ZDB-PUB-170912-8
Date
2017
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   246(12): 992-1000 (Journal)
Registered Authors
Du, Shao Jun (Jim), Rotllant, Josep
Keywords
cardiac muscle, mef2, muscle-specificity, myod, skeletal muscle, transgenic zebrafish
MeSH Terms
  • Gene Expression Regulation, Developmental
  • Zebrafish Proteins*/genetics
  • Zebrafish Proteins*/metabolism
  • Animals
  • Musculoskeletal Development/physiology*
  • Transcription, Genetic/physiology*
  • Embryo, Nonmammalian/embryology*
  • Promoter Regions, Genetic/physiology*
  • Zebrafish*/embryology
  • Zebrafish*/genetics
  • Nuclear Proteins*/genetics
  • Nuclear Proteins*/metabolism
(all 12)
PubMed
28891223 Full text @ Dev. Dyn.
Abstract
Background Mustn1 is a specific musculoskeletal protein that plays a critical role in myogenesis and chondrogenesis in vertebrates. Whole-mount in situ hybridization revealed that mustn1b mRNAs are specifically expressed in skeletal and cardiac muscles in zebrafish embryos. However, the precise function and the regulatory elements required for its muscle-specific expression are largely unknown.
Results The purpose of this study was to explore and uncover the target genomic regions that regulate mustn1b gene expression by in-vivo functional characterization of the mustn1b promoter. We report here stable expression analyses of eGFP from fluorescent transgenic reporter zebrafish line containing a 0.8kb_mustn1b-Tol2-eGFP construct. eGFP expression was specifically found in the skeletal and cardiac muscle tissues. We show that reporter zebrafish lines generated replicate the endogenous mustn1b expression pattern in early zebrafish embryos. Specific site direct-mutagenesis analysis revealed that promoter activity resides in two annotated genomic regulatory regions each one corresponding to a specific functional transcription factor binding site.
Conclusions Our data indicates that mustn1b is specifically expressed in skeletal and cardiac muscle tissues and its muscle specificity is controlled by the 0.2 kb promoter and flanking sequences and in vivo regulated by the action of two sequence-specific families of transcription factors (TFs). This article is protected by copyright. All rights reserved.
Genes / Markers
Figures
Figure Gallery (5 images)
Show all Figures
Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
iim01TgTransgenic Insertion
    mn0067GtTransgenic Insertion
    1 - 2 of 2
    Show
    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    No data available
    Fish
    Fish
    iim01Tg/iim01Tg (TU)
    myom3mn0067Gt/mn0067Gt; iim01Tg/iim01Tg (TU)
    1 - 2 of 2
    Show
    Antibodies
    No data available
    Orthology
    No data available
    Engineered Foreign Genes
    Marker Marker Type Name
    EGFPEFGEGFP
    GFPEFGGFP
    mRFP1EFGmRFP1
    1 - 3 of 3
    Show
    Mapping
    No data available
    Your Input Welcome
    We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
    Citation
    Suarez-Bregua, P., Chien, C.J., Megias, M., Du, S.D., Rotllant, J. (2017) Promoter architecture and transcriptional regulation of musculoskeletal embryonic nuclear protein 1b (mustn1b) gene in zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 246(12):992-1000.