ZFIN ID: ZDB-PUB-170825-10
Zebrafish nephrosin helps host defence against Escherichia coli infection.
Di, Q., Lin, Q., Huang, Z., Chi, Y., Chen, X., Zhang, W., Zhang, Y.
Date: 2017
Source: Open Biology   7(8): (Journal)
Registered Authors: Chen, Xiaohui, Chi, Yali, Di, Qianqian, Huang, Zhibin, Lin, Qing, Zhang, Wenqing, Zhang, Yiyue
Keywords: infection, nephrosin, neutrophil, zebrafish
MeSH Terms:
  • Animals
  • CRISPR-Cas Systems
  • Disease Models, Animal
  • Embryo, Nonmammalian/immunology
  • Embryo, Nonmammalian/metabolism
  • Embryo, Nonmammalian/microbiology
  • Escherichia coli Infections/genetics
  • Escherichia coli Infections/immunology
  • Escherichia coli Infections/prevention & control*
  • Gene Knockdown Techniques
  • Metalloendopeptidases/genetics*
  • Metalloendopeptidases/metabolism
  • Mutation
  • Neutrophils/metabolism*
  • Zebrafish
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed: 28835569 Full text @ Open Biol.
Neutrophils play important roles in innate immunity and are mainly dependent on various enzyme-containing granules to kill engulfed microorganisms. Zebrafish nephrosin (npsn) is specifically expressed in neutrophils; however, its function is largely unknown. Here, we generated an npsn mutant (npsnsmu5 ) via CRISPR/Cas9 to investigate the in vivo function of Npsn. The overall development and number of neutrophils remained unchanged in npsn-deficient mutants, whereas neutrophil antibacterial function was defective. Upon infection with Escherichia coli, the npsnsmu5 mutants exhibited a lower survival rate and more severe bacterial burden, as well as augmented inflammatory response to challenge with infection when compared with wild-type embryos, whereas npsn-overexpressing zebrafish exhibited enhanced host defence against E. coli infection. These findings demonstrated that zebrafish Npsn promotes host defence against bacterial infection. Furthermore, our findings suggested that npsn-deficient and -overexpressing zebrafish might serve as effective models of in vivo innate immunity.