PUBLICATION
Manipulating leukocyte interactions in vivo through optogenetic chemokine release
- Authors
- Sarris, M., Olekhnovitch, R., Bousso, P.
- ID
- ZDB-PUB-170809-13
- Date
- 2016
- Source
- Blood 127: e35-41 (Journal)
- Registered Authors
- Sarris, Milka
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cell Communication*/genetics
- Chemokines
- Chemotactic Factors
- Chemotaxis, Leukocyte/genetics
- Embryo, Nonmammalian
- HEK293 Cells
- Humans
- Leukocytes/physiology*
- Mice
- Mice, Inbred BALB C
- Mice, Inbred C57BL
- Mice, Transgenic
- Optogenetics
- Signal Transduction
- Zebrafish
- PubMed
- 27057000 Full text @ Blood
Citation
Sarris, M., Olekhnovitch, R., Bousso, P. (2016) Manipulating leukocyte interactions in vivo through optogenetic chemokine release. Blood. 127:e35-41.
Abstract
Light-mediated release of signaling ligands, such as chemoattractants, growth factors, and cytokines is an attractive strategy for investigation and therapeutic targeting of leukocyte communication and immune responses. We introduce a versatile optogenetic method to control ligand secretion, combining UV-conditioned endoplasmic reticulum-to-Golgi trafficking and a furin-processing step. As proof of principle, we achieved light-triggered chemokine secretion and demonstrated that a brief pulse of chemokine release can mediate a rapid flux of leukocyte contacts with target cells in vitro and in vivo. This approach opens new possibilities for dynamic investigation of leukocyte communication in vivo and may confer the potential to control the local release of soluble mediators in the context of immune cell therapies.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping