PUBLICATION
New photic stimulating system with white light-emitting diodes to elicit electroretinograms from zebrafish larvae
- Authors
- Matsubara, H., Matsui, Y., Miyata, R., Nishimura, Y., Yamamoto, T., Tanaka, T., Kondo, M.
- ID
- ZDB-PUB-170802-24
- Date
- 2017
- Source
- Documenta ophthalmologica. Advances in ophthalmology 135(2): 147-154 (Journal)
- Registered Authors
- Tanaka, Toshio
- Keywords
- Electroretinography (ERG), Light stimulator, Light-emitting diodes (LED), Retina, Visual system, Zebrafish
- MeSH Terms
-
- Animals
- Color Vision
- Electroretinography/methods*
- Larva/physiology
- Night Vision
- Photic Stimulation/instrumentation*
- Reproducibility of Results
- Retina/physiology*
- Zebrafish/physiology*
- PubMed
- 28756596 Full text @ Doc. Ophthalmol.
Citation
Matsubara, H., Matsui, Y., Miyata, R., Nishimura, Y., Yamamoto, T., Tanaka, T., Kondo, M. (2017) New photic stimulating system with white light-emitting diodes to elicit electroretinograms from zebrafish larvae. Documenta ophthalmologica. Advances in ophthalmology. 135(2):147-154.
Abstract
Purpose The zebrafish is an established animal model commonly used in biological, neuroscience, and genetic research. We have developed a new light stimulating system using white light-emitting diodes (LEDs) to elicit ERGs from zebrafish larvae. The purpose of this study was to record full-field ERGs and to evaluate the inter-trial reliability of the ERGs recorded with our system from zebrafish larvae.
Methods The stimulating device used white LEDs that were attached to a stereomicroscope, and the location of the recording electrode on the cornea could be monitored while the eye was being stimulated. Full-field scotopic and photopic ERGs were recorded from larvae at the age of 5-7 days post-fertilization (dpf). Intensity-response curves were constructed from the ERGs. Inter-trial reliability of the ERGs recorded by our system was evaluated.
Results This stimulating system could be used for efficient and reliable ERG recordings from 5-7 dpf larvae. The amplitudes, implicit times, and the waveforms of the scotopic and photopic ERGs were similar to those reported in earlier studies. Inter-trial reliability of the amplitudes of the photopic ERG b-waves was excellent with an intra-class correlation coefficient of 0.98.
Conclusion We conclude that this new light stimulation system using white LEDs attached to a stereomicroscope will be helpful in recording reliable ERGs from zebrafish larvae.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping