PUBLICATION
Label-free photoacoustic imaging of the cardio-cerebrovascular development in the embryonic zebrafish
- Authors
- Chen, Q., Jin, T., Qi, W., Mo, X., Xi, L.
- ID
- ZDB-PUB-170726-18
- Date
- 2017
- Source
- Biomedical Optics Express 8: 2359-2367 (Journal)
- Registered Authors
- Mo, Xianming
- Keywords
- (110.5120) Photoacoustic imaging, (170.6900) Three-dimensional microscopy
- MeSH Terms
- none
- PubMed
- 28736676 Full text @ Biomed. Opt. Express
Citation
Chen, Q., Jin, T., Qi, W., Mo, X., Xi, L. (2017) Label-free photoacoustic imaging of the cardio-cerebrovascular development in the embryonic zebrafish. Biomedical Optics Express. 8:2359-2367.
Abstract
Zebrafish play an important role in biology, pharmacology, toxicology, and medicine. The cardio-cerebrovascular development of zebrafish is particularly critical to understand both brain disorders and cardiovascular diseases in human. In this paper, we applied optical resolution photoacoustic microscopy (ORPAM) to image the whole-body vasculature of the embryonic zebrafish with a special focus on the development of the cardio-cerebrovascular system. Using the intrinsic optical absorption contrast of the embryo, we successfully visualized the formation of the cardio-cerebrovascular network in high-resolution using a 10 × objective, and monitored the whole-body vascular development using a 4 × objective. In addition, we evaluated the impact of the eggshell and pigment inhibitor on the image quality. Our results suggest that ORPAM is capable of studying the cardio-cerebrovascular development of zebrafish in the embryonic stage, and thus has the potential to investigate the cardiovascular and cerebrovascular diseases of human in the future.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping