PUBLICATION

Harmless effects of argon plasma on caudal fin regeneration and embryogenesis of zebrafish: novel biological approaches for safe medical applications of bioplasma

Authors
Nam, M.K., Kim, G.Y., Yun, S.E., Jang, J.Y., Kim, Y.H., Choi, E.H., Kang, S., Rhim, H.
ID
ZDB-PUB-170715-6
Date
2017
Source
Experimental & molecular medicine   49: e355 (Journal)
Registered Authors
Keywords
Biological techniques, Therapeutics
MeSH Terms
  • beta Catenin/metabolism
  • Zebrafish Proteins/metabolism
  • Argon/adverse effects*
  • Cells, Cultured
  • Argon Plasma Coagulation/adverse effects*
  • Wnt Proteins/metabolism
  • Cytoskeletal Proteins/metabolism
  • Microscopy, Confocal
  • Models, Animal
  • Plasma Gases/adverse effects*
  • Zebrafish
  • Animal Fins
  • Animals
  • Fibroblasts/metabolism
  • Regeneration*
  • Embryonic Development*
  • Microscopy, Fluorescence
(all 17)
PubMed
28706297 Full text @ Exp. Mol. Med.
Abstract
The argon plasma jet (Ar-PJ) is widely used in medical fields such as dermatology and dentistry, and it is considered a promising tool for cancer therapy. However, the in vivo effects of Ar-PJ for medical uses have not yet been investigated, and there are no biological tools to determine the appropriate clinical dosages of Ar-PJ. In this study, we used the caudal fin and embryo of zebrafish as novel in vivo tools to evaluate the biosafety of Ar-PJ. Typically, Ar-PJ is known to induce cell death in two-dimensional (2D) cell culture systems. By contrast, no detrimental effects of Ar-PJ were shown in our 3D zebrafish systems composed of 2D cells. The Ar-PJ-treated caudal fins grew by an average length of 0.7 mm, similar to the length of the normally regenerating fins. Remarkably, Ar-PJ did not affect the expression patterns of Wnt8a and β-Catenin, which play important roles in fin regeneration. In the embryo system, 85% of the Ar-PJ-treated embryos hatched, and the lateral length of these embryos was ~3.3 mm, which are equivalent to the lengths of normal embryos. In particular, vasculogenesis, which is the main cellular process during tissue regeneration and embryogenesis, occurred normally under the Ar-PJ dose used in this study. Therefore, our biosafety evaluation tools that use living model systems can be used to provide an experimental guideline to determine the clinically safe dosage of Ar-PJ.
Genes / Markers
Figures
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Phenotype
No data available
Mutations / Transgenics
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Human Disease / Model
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Sequence Targeting Reagents
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Fish
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Antibodies
Name Type Antigen Genes Isotypes Host Organism
Ab3-ctnnb1monoclonalIgG1Mouse
1 - 1 of 1
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Citation
Nam, M.K., Kim, G.Y., Yun, S.E., Jang, J.Y., Kim, Y.H., Choi, E.H., Kang, S., Rhim, H. (2017) Harmless effects of argon plasma on caudal fin regeneration and embryogenesis of zebrafish: novel biological approaches for safe medical applications of bioplasma. Experimental & molecular medicine. 49:e355.