PUBLICATION
Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation
- Authors
- Krens, S.F.G., Veldhuis, J.H., Barone, V., Čapek, D., Maître, J.L., Brodland, G.W., Heisenberg, C.P.
- ID
- ZDB-PUB-170518-6
- Date
- 2017
- Source
- Development (Cambridge, England) 144: 1798-1806 (Journal)
- Registered Authors
- Barone, Vanessa, Heisenberg, Carl-Philipp, Krens, S. F. Gabby, Maître, Jean-Léon
- Keywords
- Cell internalization, Gastrulation, Tissue surface tension, Zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Body Patterning*
- Cell Movement*
- Embryo, Nonmammalian
- Extracellular Fluid/chemistry*
- Gastrulation/physiology*
- Mesoderm/chemistry
- Mesoderm/cytology
- Mesoderm/embryology
- Osmolar Concentration
- Stem Cells/chemistry*
- Stem Cells/cytology
- Stem Cells/physiology*
- Surface Tension
- Zebrafish/embryology*
- PubMed
- 28512197 Full text @ Development
Citation
Krens, S.F.G., Veldhuis, J.H., Barone, V., Čapek, D., Maître, J.L., Brodland, G.W., Heisenberg, C.P. (2017) Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation. Development (Cambridge, England). 144:1798-1806.
Abstract
The segregation of different cell types into distinct tissues is a fundamental process in metazoan development. Differences in cell adhesion and cortex tension are commonly thought to drive cell sorting by regulating tissue surface tension (TST). However, the role that differential TST plays in cell segregation within the developing embryo is as yet unclear. Here, we have analyzed the role of differential TST for germ layer progenitor cell segregation during zebrafish gastrulation. Contrary to previous observations that differential TST drives germ layer progenitor cell segregation in vitro, we show that germ layers display indistinguishable TST within the gastrulating embryo, arguing against differential TST driving germ layer progenitor cell segregation in vivo We further show that the osmolarity of the interstitial fluid (IF) is an important factor that influences germ layer TST in vivo, and that lower osmolarity of the IF compared with standard cell culture medium can explain why germ layers display differential TST in culture but not in vivo Finally, we show that directed migration of mesendoderm progenitors is required for germ layer progenitor cell segregation and germ layer formation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping