PUBLICATION
Antibody Uptake Assay in the Embryonic Zebrafish Forebrain to Study Notch Signaling Dynamics in Neural Progenitor Cells In Vivo
- Authors
- Tong, K., Wagle, M., Guo, S.
- ID
- ZDB-PUB-170424-1
- Date
- 2017
- Source
- Methods in molecular biology (Clifton, N.J.) : (Chapter)
- Registered Authors
- Guo, Su, Wagle, Mahendra
- Keywords
- Antibody uptake assay, Asymmetric cell division, Live imaging, Notch signaling, Radial glia, Stem cell, Zebrafish
- MeSH Terms
-
- Animals
- Antibodies/immunology
- Antibodies/metabolism*
- Asymmetric Cell Division*
- Cells, Cultured
- Gene Expression Regulation, Developmental
- Luminescent Proteins/metabolism
- Neural Stem Cells/cytology*
- Neural Stem Cells/metabolism
- Neuroglia/cytology*
- Neuroglia/metabolism
- Prosencephalon/cytology*
- Prosencephalon/metabolism
- Receptors, Notch/immunology
- Receptors, Notch/metabolism*
- Zebrafish/embryology*
- Zebrafish/metabolism
- Zebrafish Proteins/immunology
- Zebrafish Proteins/metabolism
- PubMed
- 28434166 Full text @ Meth. Mol. Biol.
Citation
Tong, K., Wagle, M., Guo, S. (2017) Antibody Uptake Assay in the Embryonic Zebrafish Forebrain to Study Notch Signaling Dynamics in Neural Progenitor Cells In Vivo. Methods in molecular biology (Clifton, N.J.). .
Abstract
Stem cells can generate cell fate heterogeneity through asymmetric cell division (ACD). ACD derives from the asymmetric segregation of fate-determining molecules and/or organelles in the dividing cell. Radial glia in the embryonic zebrafish forebrain are an excellent model for studying the molecular mechanisms regulating ACD of stem cells in vertebrates, especially for live imaging concerning in vivo molecular and cellular dynamics. Due to the current difficulty in expressing fluorescent reporter-tagged proteins at physiological levels in zebrafish for live imaging, we have developed an antibody uptake assay to label proteins in live embryonic zebrafish forebrain with high specificity. DeltaD is a transmembrane ligand in Notch signaling pathway in the context of ACD of radial glia in zebrafish. By using this assay, we have successfully observed the in vivo dynamics of DeltaD for studying ACD of radial glia in the embryonic zebrafish forebrain.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping