PUBLICATION

Chemical profiling analysis of Maca using UHPLC-ESI-Orbitrap MS coupled with UHPLC-ESI-QqQ MS and the neuroprotective study on its active ingredients

Authors
Zhou, Y., Li, P., Brantner, A., Wang, H., Shu, X., Yang, J., Si, N., Han, L., Zhao, H., Bian, B.
ID
ZDB-PUB-170318-2
Date
2017
Source
Scientific Reports   7: 44660 (Journal)
Registered Authors
Yang, Jian, Zhao, Haiyu
Keywords
Classical conditioning, Mass spectrometry
MeSH Terms
  • Animals
  • Lepidium/chemistry*
  • Neuroprotective Agents/chemistry
  • Neuroprotective Agents/pharmacology*
  • Limit of Detection
  • Biological Products/chemistry
  • Biological Products/pharmacology
  • Dopaminergic Neurons/drug effects
  • Dopaminergic Neurons/metabolism
  • Dopaminergic Neurons/pathology
  • Cholinesterase Inhibitors/pharmacology
  • Zebrafish
  • Chromatography, High Pressure Liquid/methods*
  • Spectrometry, Mass, Electrospray Ionization*
(all 14)
PubMed
28304399 Full text @ Sci. Rep.
Abstract
Lepidium meyenii (Maca), originated from Peru, has been cultivated widely in China as a popular health care food. However, the chemical and effective studies of Maca were less in-depth, which restricted its application seriously. To ensure the quality of Maca, a feasible and accurate strategy was established. One hundred and sixty compounds including 30 reference standards were identified in 6 fractions of methanol extract of Maca by UHPLC-ESI-Orbitrap MS. Among them, 15 representative active compounds were simultaneously determined in 17 samples by UHPLC-ESI-QqQ MS. The results suggested that Maca from Yunnan province was the potential substitute for the one from Peru. Meanwhile, the neuroprotective effects of Maca were investigated. Three fractions and two pure compounds showed strong activities in the 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP)-induced zebrafish model. Among them, 80% methanol elution fraction (Fr5) showed significant neuroprotective activity, followed by 100% part (Fr6). The inhibition of acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) was a possible mechanism of its neuroprotective effect.
Genes / Markers
Figures
Figure Gallery (1 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
WTchemical treatment: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridineAdult
WTchemical treatment: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridineAdult
WTchemical treatment: N-benzylacetamides, chemical treatment: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridineAdult
WTchemical treatment: N-benzylacetamides, chemical treatment: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridineAdult
WTchemical treatment: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine, chemical treatment: N-benzylhexadecanamideAdult
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Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
No data available
Fish
Fish
WT
1 - 1 of 1
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Antibodies
Name Type Antigen Genes Isotypes Host Organism
Ab3-thmonoclonalIgG1Mouse
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Orthology
No data available
Engineered Foreign Genes
No data available
Mapping
No data available
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Citation
Zhou, Y., Li, P., Brantner, A., Wang, H., Shu, X., Yang, J., Si, N., Han, L., Zhao, H., Bian, B. (2017) Chemical profiling analysis of Maca using UHPLC-ESI-Orbitrap MS coupled with UHPLC-ESI-QqQ MS and the neuroprotective study on its active ingredients. Scientific Reports. 7:44660.