PUBLICATION

Enhancing the anti-glioma therapy of doxorubicin by honokiol with biodegradable self-assembling micelles through multiple evaluations

Authors
Gao, X., Yu, T., Xu, G., Guo, G., Liu, X., Hu, X., Wang, X., Liu, Y., Mao, Q., You, C., Zhou, L.
ID
ZDB-PUB-170228-3
Date
2017
Source
Scientific Reports   7: 43501 (Journal)
Registered Authors
Keywords
Chemotherapy, CNS cancer, Experimental models of disease
MeSH Terms
  • Animals
  • Antineoplastic Agents/administration & dosage
  • Antineoplastic Agents/pharmacology*
  • Apoptosis/drug effects
  • Biphenyl Compounds/administration & dosage
  • Biphenyl Compounds/pharmacology*
  • Cell Line, Tumor
  • Cell Proliferation/drug effects
  • Disease Models, Animal
  • Doxorubicin/administration & dosage
  • Doxorubicin/pharmacology*
  • Drug Carriers*/chemistry
  • Drug Liberation
  • Drug Synergism
  • Humans
  • Lignans/administration & dosage
  • Lignans/pharmacology*
  • Micelles*
  • Nanoparticles/chemistry
  • Nanoparticles/ultrastructure
  • Neovascularization, Pathologic/drug therapy
  • Polyesters/chemistry
  • Polyethylene Glycols/chemistry
  • X-Ray Diffraction
  • Xenograft Model Antitumor Assays
  • Zebrafish
PubMed
28240249 Full text @ Sci. Rep.
Abstract
Combination chemotherapy is an important protocol in glioma therapy and honokiol shows synergistic anticancer effects with doxorubicin. In this paper, honokiol (HK) and doxorubicin (Dox) co-loaded Methoxy poly(ethylene glycol)-poly(ε-caprolactone) (MPEG-PCL) nanoparticles were prepared with a assembly method. The particle size (about 34 nm), morphology, X-ray Powder Diffraction (XRD), in vitro release profile, cytotoxicity and cell proliferation effects were studied in detail. The results indicated that honokiol and doxorubicin could be efficiently loaded into MPEG-PCL nanoparticles simultaneously, and could be released from the micelles in an extended period in vitro. In addition, honokiol and doxorubicin loaded in MPEG-PCL nanoparticles could efficiently suppress glioma cell proliferation and induce cell apoptosis in vitro. Furthermore, Dox-HK-MPEG-PCL micelles inhibited glioma growth more significantly than Dox-MPEG-PCL and HK-MPEG-PCL in both nude mice and zebrafish tumor models. Immunohistochemical analysis indicated that DOX-HK-MPEG-PCL micelles improved Dox's anti-tumor effect by enhancing tumor cell apoptosis, suppressing tumor cell proliferation, and inhibiting angiogenesis. Our data suggest that Dox-HK-MPEG-PCL micelles have the potential to be applied clinically in glioma therapy.
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