Flotillins control zebrafish epiboly through their role in cadherin-mediated cell-cell adhesion
- Morris, E.A., Bodin, S., Delaval, B., Comunale, F., Georget, V., Costa, M.L., Lutfalla, G., Gauthier-Rouvière, C.
- Biology of the cell 109(5): 210-221 (Journal)
- Registered Authors
- Costa, Manoel Luis, Lutfalla, Georges
- E-cadherin, Flotillins, Gastrulation, Zebrafish
- MeSH Terms
- Cell Adhesion
- Cell Communication
- Cell Movement*
- Gene Knockdown Techniques
- Membrane Proteins/metabolism*
- Zebrafish Proteins/metabolism*
- beta Catenin/metabolism
- 28225561 Full text @ Biol. Cell
Morris, E.A., Bodin, S., Delaval, B., Comunale, F., Georget, V., Costa, M.L., Lutfalla, G., Gauthier-Rouvière, C. (2017) Flotillins control zebrafish epiboly through their role in cadherin-mediated cell-cell adhesion. Biology of the cell. 109(5):210-221.
Zebrafish gastrulation and particularly epiboly that involves coordinated movements of several cell layers is a dynamic process for which regulators remain to be identified. We show here that Flotillin 1 and 2, ubiquitous and highly conserved proteins, are required for epiboly. Flotillins knockdown compromised embryo survival, strongly delayed epiboly and impaired deep cell radial intercalation and directed collective migration without affecting enveloping layer cell movement. At the molecular level, we identified that Flotillins are required for the formation of E-cadherin-mediated cell-cell junctions. These results provide the first in vivo evidence that Flotillins regulate E-cadherin-mediated cell-cell junctions to allow epiboly progression.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes