ZFIN ID: ZDB-PUB-170223-1
Flotillins control zebrafish epiboly through their role in cadherin-mediated cell-cell adhesion
Morris, E.A., Bodin, S., Delaval, B., Comunale, F., Georget, V., Costa, M.L., Lutfalla, G., Gauthier-Rouvière, C.
Date: 2017
Source: Biology of the cell   109(5): 210-221 (Journal)
Registered Authors: Costa, Manoel Luis, Lutfalla, Georges
Keywords: E-cadherin, Flotillins, Gastrulation, Zebrafish
MeSH Terms:
  • Animals
  • Cadherins/metabolism*
  • Cell Adhesion
  • Cell Communication
  • Cell Movement*
  • Gene Knockdown Techniques
  • Membrane Proteins/metabolism*
  • Zebrafish/metabolism*
  • Zebrafish Proteins/metabolism*
  • beta Catenin/metabolism
PubMed: 28225561 Full text @ Biol. Cell
Zebrafish gastrulation and particularly epiboly that involves coordinated movements of several cell layers is a dynamic process for which regulators remain to be identified. We show here that Flotillin 1 and 2, ubiquitous and highly conserved proteins, are required for epiboly. Flotillins knockdown compromised embryo survival, strongly delayed epiboly and impaired deep cell radial intercalation and directed collective migration without affecting enveloping layer cell movement. At the molecular level, we identified that Flotillins are required for the formation of E-cadherin-mediated cell-cell junctions. These results provide the first in vivo evidence that Flotillins regulate E-cadherin-mediated cell-cell junctions to allow epiboly progression.