PUBLICATION

Cell division and cadherin-mediated adhesion regulate lens epithelial cell movement in zebrafish

Authors
Mochizuki, T., Luo, Y.J., Tsai, H.F., Hagiwara, A., Masai, I.
ID
ZDB-PUB-170216-5
Date
2017
Source
Development (Cambridge, England)   144: 708-719 (Journal)
Registered Authors
Masai, Ichiro, Mochizuki, Toshiaki
Keywords
Cadherin, Cell movement, Epithelium, Lens, Tension, Zebrafish
MeSH Terms
  • Lens, Crystalline/cytology
  • Lens, Crystalline/embryology*
  • Zebrafish Proteins/metabolism*
  • Green Fluorescent Proteins/metabolism
  • Cell Lineage
  • Animals
  • Oligonucleotides, Antisense/metabolism
  • Animals, Genetically Modified
  • Zebrafish
  • Cell Movement
  • Epithelial Cells/cytology*
  • Cadherins/metabolism*
  • Cell Division
(all 13)
PubMed
28196805 Full text @ Development
Abstract
In vertebrates, lens epithelial cells cover the anterior half of the lens fiber core. During development, lens epithelial cells proliferate, move posteriorly and differentiate into lens fiber cells after passing through the equator. To elucidate the mechanisms underlying lens epithelial cell movement, we conducted time-lapse imaging of zebrafish lens epithelium. Lens epithelial cells do not intermingle but maintain their relative positions during development. Cell division induces epithelial rearrangement, which subsequently promotes cell movement towards the equator. These data suggest that cell division is the major driving force for cell movement. In zebrafish, E-cadherin is expressed in lens epithelium, whereas N-cadherin is required for lens fiber growth. E-cadherin reduced lens epithelial cell movement, whereas N-cadherin enhanced it. Laser ablation experiments revealed that lens epithelium is governed by pulling tension, which is modulated by these cadherins. Thus, cell division and cadherin-mediated adhesion regulate lens epithelial cell movement via modulation of epithelial tension.
Genes / Markers
Figures
Figure Gallery (17 images) / 2
Show all Figures
Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
GFPcdh1rk3/rk3; kca66Tg; kca6Tg; oki011Tglight ablation: lens epitheliumLong-pecIFL
GFPcdh1rk3/rk3; kca66Tg; kca6Tg; oki011TgcontrolPrim-15 to High-pecIFL
GFPkca66Tg; kca6Tg; oki011Tglight ablation: lens epitheliumLong-pecIFL
GFPkca66Tg; kca6Tg; oki011TgcontrolPrim-15 to High-pecIFL
GFPkca66Tg; kca6Tg; oki011Tg + MO1-cdh2light ablation: lens epitheliumLong-pecIFL
GFPkca66Tg; kca6Tg; oki011Tg + MO1-cdh2controlPrim-15 to High-pecIFL
mCherrycdh1rk3/rk3; kca66Tg; kca6Tg; oki011TgcontrolPrim-15 to High-pecIFL
mCherrycdh1rk3/rk3; kca66Tg; kca6Tg; oki011Tglight ablation: lens epitheliumLong-pecIFL
mCherrykca66Tg; kca6Tg; oki011Tglight ablation: lens epitheliumLong-pecIFL
mCherrykca66Tg; kca6Tg; oki011TgcontrolPrim-15 to High-pecIFL
1 - 10 of 12
Show
Phenotype
Fish Conditions Stage Phenotype Figure
cdh1rk3/rk3; kca66Tg; kca6Tg; oki011TgcontrolPrim-15 to High-pec
cdh1rk3/rk3; kca66Tg; kca6Tg; oki011TgcontrolPrim-15 to High-pec
cdh1rk3/rk3; kca66Tg; kca6Tg; oki011TgcontrolLong-pec
cdh1rk3/rk3; kca66Tg; kca6Tg; oki011Tglight ablation: lens epitheliumLong-pec
cdh1rk3/rk3; kca66Tg; kca6Tg; oki011Tglight ablation: lens epitheliumLong-pec
kca66Tg; kca6Tg; oki011Tg + MO1-cdh2controlPrim-15 to High-pec
kca66Tg; kca6Tg; oki011Tg + MO1-cdh2controlPrim-15 to High-pec
kca66Tg; kca6Tg; oki011Tg + MO1-cdh2light ablation: lens epitheliumLong-pec
kca66Tg; kca6Tg; oki011Tg + MO1-cdh2light ablation: lens epitheliumLong-pec
1 - 9 of 9
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
e2212TgTransgenic Insertion
    kca6TgTransgenic Insertion
      kca66TgTransgenic Insertion
        oki011TgTransgenic Insertion
          oki049TgTransgenic Insertion
            rk3
              		Point Mutation
              1 - 6 of 6
              Show
              Human Disease / Model
              No data available
              Sequence Targeting Reagents
              Target Reagent Reagent Type
              cdh1MO3-cdh1MRPHLNO
              cdh2MO1-cdh2MRPHLNO
              1 - 2 of 2
              Show
              Fish
              Fish
              cdh1rk3/rk3; kca66Tg; kca6Tg; oki011Tg
              kca66Tg; kca6Tg; oki011Tg
              kca66Tg; kca6Tg; oki011Tg + MO1-cdh2
              1 - 3 of 3
              Show
              Antibodies
              Orthology
              No data available
              Engineered Foreign Genes
              Marker Marker Type Name
              EGFPEFGEGFP
              GFPEFGGFP
              mCherryEFGmCherry
              1 - 3 of 3
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              Mapping
              No data available
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              Citation
              Mochizuki, T., Luo, Y.J., Tsai, H.F., Hagiwara, A., Masai, I. (2017) Cell division and cadherin-mediated adhesion regulate lens epithelial cell movement in zebrafish. Development (Cambridge, England). 144:708-719.