PUBLICATION
The Zebrafish Anillin-eGFP Reporter Marks Late Dividing Retinal Precursors and Stem Cells Entering Neuronal Lineages
- Authors
- Cepero Malo, M., Duchemin, A.L., Guglielmi, L., Patzel, E., Sel, S., Auffarth, G.U., Carl, M., Poggi, L.
- ID
- ZDB-PUB-170121-1
- Date
- 2017
- Source
- PLoS One 12: e0170356 (Journal)
- Registered Authors
- Carl, Matthias, Duchemin, Anne-Laure, Patzel, Eva, Poggi, Lucia
- Keywords
- Retina, Cell cycle and cell division, Zebrafish, Precursor cells, Photoreceptors, Embryos, Cytokinesis, Mitosis
- MeSH Terms
-
- Contractile Proteins/genetics*
- Genes, Reporter*
- Cell Division
- Green Fluorescent Proteins/genetics*
- Neurons/cytology*
- Retina/cytology*
- Cell Lineage
- Zebrafish/genetics*
- Animals
- PubMed
- 28107513 Full text @ PLoS One
Citation
Cepero Malo, M., Duchemin, A.L., Guglielmi, L., Patzel, E., Sel, S., Auffarth, G.U., Carl, M., Poggi, L. (2017) The Zebrafish Anillin-eGFP Reporter Marks Late Dividing Retinal Precursors and Stem Cells Entering Neuronal Lineages. PLoS One. 12:e0170356.
Abstract
Monitoring cycling behaviours of stem and somatic cells in the living animal is a powerful tool to better understand tissue development and homeostasis. The tg(anillin:anillin-eGFP) transgenic line carries the full-length zebrafish F-actin binding protein Anillin fused to eGFP from a bacterial artificial chromosome (BAC) containing Anillin cis-regulatory sequences. Here we report the suitability of the Anillin-eGFP reporter as a direct indicator of cycling cells in the late embryonic and post-embryonic retina. We show that combining the anillin:anillin-eGFP with other transgenes such as ptf1a:dsRed and atoh7:gap-RFP allows obtaining spatial and temporal resolution of the mitotic potentials of specific retinal cell populations. This is exemplified by the analysis of the origin of the previously reported apically and non-apically dividing late committed precursors of the photoreceptor and horizontal cell layers.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping