PUBLICATION
Microglial Activation by Genetically Targeted Conditional Neuronal Ablation in the Zebrafish
- Authors
- Oosterhof, N., Kuil, L.E., van Ham, T.J.
- ID
- ZDB-PUB-170108-2
- Date
- 2017
- Source
- Methods in molecular biology (Clifton, N.J.) 1559: 377-390 (Chapter)
- Registered Authors
- van Ham, Tjakko
- Keywords
- Aging, Alzheimer’s disease, Brain, Immunohistochemistry, Live imaging, Macrophages, Microglia, Neurodegeneration, Nitroreductase, Phagocytosis, Zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Apoptosis/genetics*
- Brain/metabolism
- Brain/ultrastructure*
- Disease Models, Animal
- Gene Expression
- Genes, Reporter
- Green Fluorescent Proteins/genetics
- Green Fluorescent Proteins/metabolism
- Humans
- Immunohistochemistry/methods
- Larva/genetics
- Larva/metabolism
- Larva/ultrastructure*
- Luminescent Proteins/genetics
- Luminescent Proteins/metabolism
- Microglia/metabolism
- Microglia/ultrastructure*
- Microscopy, Fluorescence/methods
- Neurodegenerative Diseases/genetics
- Neurodegenerative Diseases/metabolism
- Neurodegenerative Diseases/pathology
- Neurons/metabolism
- Neurons/ultrastructure*
- Phagocytosis
- Phase Transition
- Sepharose/chemistry
- Zebrafish/genetics
- Zebrafish/metabolism
- PubMed
- 28063058 Full text @ Meth. Mol. Biol.
Citation
Oosterhof, N., Kuil, L.E., van Ham, T.J. (2017) Microglial Activation by Genetically Targeted Conditional Neuronal Ablation in the Zebrafish. Methods in molecular biology (Clifton, N.J.). 1559:377-390.
Abstract
In neurodegenerative diseases activation of immune cells is thought to play a major role. Microglia are the main immune cells of the central nervous system. When encountering disease related stimuli microglia adopt an activated phenotype that typically includes a rounded morphology. The exact role of microglia or other potentially infiltrating myeloid cells in different brain diseases is not fully understood. In this chapter we present techniques in zebrafish to induce degeneration of neurons, to activate the microglia, and to study activation phenotypes by immunohistochemistry and in vivo by fluorescence microscopic imaging.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping