PUBLICATION
The larvicide pyriproxyfen blamed during the Zika virus outbreak does not cause microcephaly in zebrafish embryos
- Authors
- Dzieciolowska, S., Larroque, A.L., Kranjec, E.A., Drapeau, P., Samarut, E.
- ID
- ZDB-PUB-170105-3
- Date
- 2017
- Source
- Scientific Reports 7: 40067 (Journal)
- Registered Authors
- Drapeau, Pierre, Samarut, Eric
- Keywords
- Developmental biology, Neuroscience
- MeSH Terms
-
- Animals
- Brain/embryology*
- Cell Count
- Disease Models, Animal
- Disease Outbreaks
- Insecticides/toxicity*
- Microcephaly/chemically induced*
- Neural Stem Cells/drug effects
- Neural Stem Cells/physiology
- Pyridines/toxicity*
- Zebrafish/embryology
- PubMed
- 28051181 Full text @ Sci. Rep.
Citation
Dzieciolowska, S., Larroque, A.L., Kranjec, E.A., Drapeau, P., Samarut, E. (2017) The larvicide pyriproxyfen blamed during the Zika virus outbreak does not cause microcephaly in zebrafish embryos. Scientific Reports. 7:40067.
Abstract
Although the zika virus (ZIKV) has now been strongly correlated with emerging cases of microcephaly in the Americas, suspicions have been raised regarding the use of pyriproxyfen, a larvicide that prevents mosquito development, in drinking water. The effects of this compound on neurodevelopment have not yet been addressed specifically in vertebrates. As a result, we aimed at addressing the effects, if any, of pyriproxyfen on neurodevelopment in the zebrafish embryo as a vertebrate model. Using zebrafish transgenic lines expressing GFP in different cell populations (elavl3 in newborn neurons, gfap and nestin in neural stem cells), we focused on the analysis of whole embryonic brain volume after confocal 3D-reconstruction and the quantification of purified neural stem cells during early neurodevelopment by FACS-cell sorting from whole in vivo embryos. Interestingly, though lethal at very high doses, pyriproxyfen did not cause brain malformation nor any significant changes in the number of observed stem cells in the developing central nervous system. Our data indicate that pyriproxyfen does not affect central nervous system development in zebrafish, suggesting that this larvicide on its own, may not be correlated with the increase in microcephaly cases reported recently.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping