ZFIN Person: Drapeau, Pierre

ZFIN ID: ZDB-PERS-970314-4

Drapeau, Pierre

Email:	p.drapeau@umontreal.ca
URL:	http://neurosciences.umontreal.ca/recherche/les-chercheurs/pierre-drapeau/
Affiliation:	Pierre Drapeau Lab
Address:	Dept. Neuroscience University of Montréal CRCHUM Tour Viger R09.482 900 Saint-Denis Montréal, Québec, Canada H3G 1A4 Canada
Country:	Canada
Phone:	514-890-8000 x31281
Fax:
ORCID ID:

BIOGRAPHY AND RESEARCH INTERESTS

As a model for the formation and function of CNS circuits, we are studying the development of the locomotor network in manipulable zebrafish embryos by combining cellular neurophysiology and molecular genetics. We record and image the activity patterns of identified neurons during development in wild-type and genetically modified embryos, including by transgenic over-expression, upon antisense knockdown and in mutations that affect locomotion. Our goal is to elucidate the molecular choreography of locomotor network formation and function. We are also using the zebrafish as a model for the validation of mutations and screening of potential therapeutics for human genes linked by genomic analyses to neurological and psychiatric developmental disorders.

PUBLICATIONS

Bose, P., Tremblay, E., Maois, C., Narasimhan, V., Armstrong, G.A.B., Liao, M., Parker, J.A., Robitaille, R., Wen, X.Y., Barden, C., Drapeau, P. (2019) The Novel Small Molecule TRVA242 Stabilizes Neuromuscular Junction Defects in Multiple Animal Models of Amyotrophic Lateral Sclerosis. Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics. 16(4):1149-1166

Samarut, E., Chalopin, D., Riché, R., Allard, M., Liao, M., Drapeau, P. (2019) Individual knock out of glycine receptor alpha subunits identifies a specific requirement of glra1 for motor function in zebrafish. PLoS One. 14:e0216159

Samarut, É., Nixon, J., Kundap, U.P., Drapeau, P., Ellis, L.D. (2019) Single and Synergistic Effects of Cannabidiol and Δ-9-Tetrahydrocannabinol on Zebrafish Models of Neuro-Hyperactivity. Frontiers in pharmacology. 10:226

Swaminathan, A., Basu, M., Bekri, A., Drapeau, P., Kundu, T.K. (2019) The Dietary Flavonoid, Luteolin, Negatively Affects Neuronal Differentiation. Frontiers in molecular neuroscience. 12:41

Bekri, A., Liao, M., Drapeau, P. (2019) Glycine Regulates Neural Stem Cell Proliferation During Development via Lnx1-Dependent Notch Signaling. Frontiers in molecular neuroscience. 12:44

Lissouba, A., Liao, M., Kabashi, E., Drapeau, P. (2018) Transcriptomic Analysis of Zebrafish TDP-43 Transgenic Lines. Frontiers in molecular neuroscience. 11:463

Bose, P., Armstrong, G.A.B., Drapeau, P. (2018) Neuromuscular junction abnormalities in a zebrafish loss-of-function model of TDP-43. Journal of neurophysiology. 121(1):285-297

Riché, R., Liao, M., Pena, I.A., Leung, K.Y., Lepage, N., Greene, N., Sarafoglou, K., Schimmenti, L.A., Drapeau, P., Samarut, É. (2018) Glycine decarboxylase deficiency-induced motor dysfunction in zebrafish is rescued by counterbalancing glycine synaptic level. JCI insight. 3(21):

Samarut, É., Swaminathan, A., Riché, R., Liao, M., Hassan-Abdi, R., Renault, S., Allard, M., Dufour, L., Cossette, P., Soussi-Yanicostas, N., Drapeau, P. (2018) γ-Aminobutyric acid receptor alpha 1 subunit loss of function causes genetic generalized epilepsy by impairing inhibitory network neurodevelopment. Epilepsia. 59(11):2061-2074

Bekri, A., Drapeau, P. (2018) Glycine Promotes the Survival of a Subpopulation of Neural Stem Cells. Frontiers in cell and developmental biology. 6:68

Swaminathan, A., Hassan-Abdi, R., Renault, S., Siekierska, A., Riché, R., Liao, M., de Witte, P.A.M., Yanicostas, C., Soussi-Yanicostas, N., Drapeau, P., Samarut, É. (2018) Non-canonical mTOR-Independent Role of DEPDC5 in Regulating GABAergic Network Development. Current biology : CB. 28(12):1924-1937.e5

Swaminathan, A., Bouffard, M., Liao, M., Ryan, S., Bennion Callister, J., Pickering-Brown, S.M., Armstrong, G.A.B., Drapeau, P. (2018) Expression of C9orf72-related dipeptides impairs motor function in a vertebrate model. Human molecular genetics. 27(10):1754-1762

Patten, S.A., Aggad, D., Martinez, J., Tremblay, E., Petrillo, J., Armstrong, G.A., La Fontaine, A., Maios, C., Liao, M., Ciura, S., Wen, X.Y., Rafuse, V., Ichida, J., Zinman, L., Julien, J.P., Kabashi, E., Robitaille, R., Korngut, L., Parker, J.A., Drapeau, P. (2017) Neuroleptics as therapeutic compounds stabilizing neuromuscular transmission in amyotrophic lateral sclerosis. JCI insight. 2(22)

Bancelin, S., Couture, C.A., Pinsard, M., Rivard, M., Drapeau, P., Légaré, F. (2017) Probing microtubules polarity in mitotic spindles in situ using Interferometric Second Harmonic Generation Microscopy. Scientific Reports. 7:6758

Dzieciolowska, S., Drapeau, P., Armstrong, G.A.B. (2017) Augmented quantal release of acetylcholine at the vertebrate neuromuscular junction following tdp-43 depletion. PLoS One. 12:e0177005

Dzieciolowska, S., Larroque, A.L., Kranjec, E.A., Drapeau, P., Samarut, E. (2017) The larvicide pyriproxyfen blamed during the Zika virus outbreak does not cause microcephaly in zebrafish embryos. Scientific Reports. 7:40067

Samarut, É., Lissouba, A., Drapeau, P. (2016) A simplified method for identifying early CRISPR-induced indels in zebrafish embryos using High Resolution Melting analysis. BMC Genomics. 17:547

Hanwell, D., Hutchinson, S.A., Collymore, C., Bruce, A.E., Louis, R., Ghalami, A., Allison, W.T., Ekker, M., Eames, B.F., Childs, S., Kurrasch, D.M., Gerlai, R., Thiele, T., Scott, I., Ciruna, B., Dowling, J.J., McFarlane, S., Huang, P., Wen, X.Y., Akimenko, M.A., Waskiewicz, A.J., Drapeau, P., Babiuk, L.A., Dragon, D., Smida, A., Buret, A.G., O'Grady, E., Wilson, J., Sowden-Plunkett, L., Tropepe, V. (2016) Restrictions on the Importation of Zebrafish into Canada Associated with Spring Viremia of Carp Virus. Zebrafish. 13 Suppl 1:S153-63

Stil, A., Drapeau, P. (2016) Neuronal labeling patterns in the spinal cord of adult transgenic Zebrafish. Developmental Neurobiology. 76(6):642-60

Gan-Or, Z., Bouslam, N., Birouk, N., Lissouba, A., Chambers, D.B., Vérièpe, J., Androschuck, A., Laurent, S.B., Rochefort, D., Spiegelman, D., Dionne-Laporte, A., Szuto, A., Liao, M., Figlewicz, D.A., Bouhouche, A., Benomar, A., Yahyaoui, M., Ouazzani, R., Yoon, G., Dupré, N., Suchowersky, O., Bolduc, F.V., Parker, J.A., Dion, P.A., Drapeau, P., Rouleau, G.A., Bencheikh, B.O. (2016) Mutations in CAPN1 Cause Autosomal-Recessive Hereditary Spastic Paraplegia. American journal of human genetics. 98:1038-1046

Samarut, E., Bekri, A., Drapeau, P. (2016) Transcriptomic Analysis of Purified Embryonic Neural Stem Cells from Zebrafish Embryos Reveals Signaling Pathways Involved in Glycine-Dependent Neurogenesis. Frontiers in molecular neuroscience. 9:22

Julien, C., Lissouba, A., Madabattula, S., Fardghassemi, Y., Rosenfelt, C., Androschuk, A., Strautman, J., Wong, C., Bysice, A., O'Sullivan, J., Rouleau, G.A., Drapeau, P., Parker, J.A., Bolduc, F.V. (2016) Conserved pharmacological rescue of hereditary spastic paraplegia-related phenotypes across model organisms. Human molecular genetics. 25(6):1088-99

Armstrong, G.A., Liao, M., You, Z., Lissouba, A., Chen, B.E., Drapeau, P. (2016) Homology Directed Knockin of Point Mutations in the Zebrafish tardbp and fus Genes in ALS Using the CRISPR/Cas9 System. PLoS One. 11:e0150188

Wang, M., De Marco, P., Merello, E., Drapeau, P., Capra, V., Kibar, Z. (2015) Role of the planar cell polarity gene Protein tyrosine kinase 7 in neural tube defects in humans. Birth defects research. Part A, Clinical and molecular teratology. 103(12):1021-7

Capo-Chichi, J.M., Boissel, S., Brustein, E., Pickles, S., Fallet-Bianco, C., Nassif, C., Patry, L., Dobrzeniecka, S., Liao, M., Labuda, D., Samuels, M.E., Hamdan, F.F., Velde, C.V., Rouleau, G.A., Drapeau, P., Michaud, J.L. (2015) Disruption of CLPB is associated with congenital microcephaly, severe encephalopathy and 3-methylglutaconic aciduria. Journal of Medical Genetics. 52(5):303-11

Patten, S.A., Margaritte-Jeannin, P., Bernard, J.C., Alix, E., Labalme, A., Besson, A., Girard, S.L., Fendri, K., Fraisse, N., Biot, B., Poizat, C., Campan-Fournier, A., Abelin-Genevois, K., Cunin, V., Zaouter, C., Liao, M., Lamy, R., Lesca, G., Menassa, R., Marcaillou, C., Letexier, M., Sanlaville, D., Berard, J., Rouleau, G.A., Clerget-Darpoux, F., Drapeau, P., Moldovan, F., Edery, P. (2015) Functional variants of POC5 identified in patients with idiopathic scoliosis. J. Clin. Invest.. 125(3):1124-8

Angers, A., Drapeau, P. (2014) Itch Is Required for Lateral Line Development in Zebrafish. PLoS One. 9:e111799

Knogler, L.D., Drapeau, P. (2014) Sensory gating of an embryonic zebrafish interneuron during spontaneous motor behaviors. Frontiers in neural circuits. 8:121

Knogler, L.D., Ryan, J., Saint-Amant, L., Drapeau, P. (2014) A hybrid electrical/chemical circuit in the spinal cord generates a transient embryonic motor behavior. The Journal of neuroscience : the official journal of the Society for Neuroscience. 34:9644-55

Patten, S.A., Armstrong, G.A., Lissouba, A., Kabashi, E., Parker, J.A., Drapeau, P. (2014) Fishing for causes and cures of motor neuron disorders. Disease models & mechanisms. 7:799-809

Armstrong, G.A., and Drapeau, P. (2013) Loss and gain of FUS function impair neuromuscular synaptic transmission in a genetic model of ALS. Human molecular genetics. 22(21):4282-4292

Vaccaro, A., Patten, S.A., Aggad, D., Julien, C., Maios, C., Kabashi, E., Drapeau, P., and Parker, J.A. (2013) Pharmacological reduction of ER stress protects against TDP-43 neuronal toxicity in vivo. Neurobiology of disease. 55:64-75

Kabashi, E., El Oussini, H., Bercier, V., Gros-Louis, F., Valdmanis, P.N., McDearmid, J., Mejier, I.A., Dion, P.A., Dupre, N., Hollinger, D., Sinniger, J., Dirrig-Grosch, S., Camu, W., Meininger, V., Loeffler, J.P., René, F., Drapeau, P., Rouleau, G.A., and Dupuis, L. (2013) Investigating the contribution of VAPB/ALS8 loss of function in amyotrophic lateral sclerosis. Human molecular genetics. 22(12):2350-60

Brustein, E., Côté, S., Ghislain, J., and Drapeau, P. (2013) Spontaneous glycine-induced calcium transients in spinal cord progenitors promote neurogenesis. Developmental Neurobiology. 73(2):168-175

Armstrong, G.A., and Drapeau, P. (2013) Calcium Channel Agonists Protect against Neuromuscular Dysfunction in a Genetic Model of TDP-43 Mutation in ALS. The Journal of neuroscience : the official journal of the Society for Neuroscience. 33(4):1741-1752

Bercier, V., Brustein, E., Liao, M., Dion, P.A., Lafrenière, R.G., Rouleau, G.A., and Drapeau, P. (2013) WNK1/HSN2 Mutation in Human Peripheral Neuropathy Deregulates KCC2 Expression and Posterior Lateral Line Development in Zebrafish (Danio rerio). PLoS Genetics. 9(1):e1003124

Côté, S., and Drapeau, P. (2012) Regulation of spinal interneuron differentiation by the paracrine action of glycine. Developmental Neurobiology. 72(2):208-214

Vaccaro, A., Patten, S.A., Ciura, S., Maios, C., Therrien, M., Drapeau, P., Kabashi, E., and Parker, J.A. (2012) Methylene Blue Protects against TDP-43 and FUS Neuronal Toxicity in C. elegans and D. rerio. PLoS One. 7(7):e42117

Patten, S.A., Jacobs-McDaniels, N.L., Zaouter, C., Drapeau, P., Albertson, R.C., and Moldovan, F. (2012) Role of Chd7 in Zebrafish: A Model for CHARGE Syndrome. PLoS One. 7(2):e31650

Bosoi, C.M., Capra, V., Allache, R., Trinh, V.Q., De Marco, P., Merello, E., Drapeau, P., Bassuk, A.G., and Kibar, Z. (2011) Identification and characterization of novel rare mutations in the planar cell polarity gene PRICKLE1 in human neural tube defects. Human Mutation. 32(12):1371-5

Kabashi, E., Bercier, V., Lissouba, A., Liao, M., Brustein, E., Rouleau, G.A, and Drapeau, P. (2011) FUS and TARDBP but Not SOD1 Interact in Genetic Models of Amyotrophic Lateral Sclerosis. PLoS Genetics. 7(8):e1002214

Kabashi, E., Brustein, E., Champagne, N., and Drapeau, P. (2011) Zebrafish models for the functional genomics of neurogenetic disorders. Biochimica et biophysica acta. Molecular basis of disease. 1812(3):335-345

Tarabeux, J., Champagne, N., Brustein, E., Hamdan, F.F., Gauthier, J., Lapointe, M., Maios, C., Piton, A., Spiegelman, D., Henrion, E., Team, S.T., Millet, B., Rapoport, J.L., Delisi, L.E., Joober, R., Fathalli, F., Fombonne, E., Mottron, L., Forget-Dubois, N., Boivin, M., Michaud, J.L., Lafrenière, R.G., Drapeau, P., Krebs, M.O., and Rouleau, G.A. (2010) De Novo Truncating Mutation in Kinesin 17 Associated with Schizophrenia. Biological psychiatry. 68(7):649-656

Kabashi, E., Champagne, N., Brustein, E., and Drapeau, P. (2010) In the swim of things: recent insights to neurogenetic disorders from zebrafish. Trends in genetics : TIG. 26(8):373-381

Reynolds, A., McDearmid, J.R., Lachance, S., Marco, P.D., Merello, E., Capra, V., Gros, P., Drapeau, P., and Kibar, Z. (2010) VANGL1 rare variants associated with neural tube defects affect convergent extension in zebrafish. Mechanisms of Development. 127(7-8):385-392

Knogler, L.D., Liao, M., and Drapeau, P. (2010) Synaptic scaling and the development of a motor network. The Journal of neuroscience : the official journal of the Society for Neuroscience. 30(26):8871-8881

Gauthier, J., Champagne, N., Lafrenière, R.G., Xiong, L., Spiegelman, D., Brustein, E., Lapointe, M., Peng, H., Côté, M., Noreau, A., Hamdan, F.F., Addington, A.M., Rapoport, J.L., Delisi, L.E., Krebs, M.O., Joober, R., Fathalli, F., Mouaffak, F., Haghighi, A.P., Néri, C., Dubé, M.P., Samuels, M.E., Marineau, C., Stone, E.A., Awadalla, P., Barker, P.A., Carbonetto, S., Drapeau, P., Rouleau, G.A. and the S2D Team. (2010) De novo mutations in the gene encoding the synaptic scaffolding protein SHANK3 in patients ascertained for schizophrenia. Proceedings of the National Academy of Sciences of the United States of America. 107(17):7863-7868

Kabashi, E., Lin, L., Tradewell, M.L., Dion, P.A., Bercier, V., Bourgouin, P., Rochefort, D., Bel Hadj, S., Durham, H.D., Vande Velde, C., Rouleau, G.A., and Drapeau, P. (2010) Gain and loss of function of ALS-related mutations of TARDBP (TDP-43) cause motor deficits in vivo. Human molecular genetics. 19(4):671-683

Suster, M.L., Kania, A., Liao, M., Asakawa, K., Charron, F., Kawakami, K., and Drapeau, P. (2009) A novel conserved evx1 enhancer links spinal interneuron morphology and cis-regulation from fish to mammals. Developmental Biology. 325(2):422-433

Montpetit, A., Côté, S., Brustein, E., Drouin, C.A., Lapointe, L., Boudreau, M., Meloche, C., Drouin, R., Hudson, T.J., Drapeau, P., and Cossette, P. (2008) Disruption of AP1S1, causing a novel neurocutaneous syndrome, perturbs development of the skin and spinal cord. PLoS Genetics. 4(12):e1000296

Chong, M., Liao, M., and Drapeau, P. (2008) The vesicular integral protein-like gene is essential for development of a mechanosensory system in zebrafish. Developmental Neurobiology. 68(12):1391-1405

Gros-Louis, F., Kriz, J., Kabashi, E., McDearmid, J., Millecamps, S., Urushitani, M., Lin, L., Dion, P., Zhu, Q., Drapeau, P., Julien, J.P., and Rouleau, G.A. (2008) Als2 mRNA splicing variants detected in KO mice rescue severe motor dysfunction phenotype in Als2 knock down zebrafish. Human molecular genetics. 17(17):2691-2702

Reynolds, A., Brustein, E., Liao, M., Mercado, A., Babilonia, E., Mount, D.B., and Drapeau, P. (2008) Neurogenic role of the depolarizing chloride gradient revealed by global overexpression of KCC2 from the onset of development. The Journal of neuroscience : the official journal of the Society for Neuroscience. 28(7):1588-1597

Chong, M., and Drapeau, P. (2007) Interaction between hindbrain and spinal networks during the development of locomotion in zebrafish. Developmental Neurobiology. 67(7):933-947

Valdmanis, P.N., Meijer, I.A., Reynolds, A., Lei, A., Macleod, P., Schlesinger, D., Zatz, M.,. Reid, E., Dion, P.A., Drapeau, P., and Rouleau, G.A. (2007) Mutations in the KIAA0196 Gene at the SPG8 Locus Cause Hereditary Spastic Paraplegia. American journal of human genetics. 80(1):152-161

McDearmid, J.R., Liao, M., and Drapeau, P. (2006) Glycine receptors regulate interneuron differentiation during spinal network development. Proceedings of the National Academy of Sciences of the United States of America. 103(25):9679-9684

McDearmid, J.R., and Drapeau, P. (2006) Rhythmic Motor Activity Evoked by NMDA in the Spinal Zebrafish Larva. Journal of neurophysiology. 95(1):401-417

Brustein, E., and Drapeau, P. (2005) Serotoninergic Modulation of Chloride Homeostasis during Maturation of the Locomotor Network in Zebrafish. The Journal of neuroscience : the official journal of the Society for Neuroscience. 25(46):10607-10616

Brustein, E., Chong, M., Holmqvist, B., and Drapeau, P. (2003) Serotonin patterns locomotor network activity in the developing zebrafish by modulating quiescent periods. Journal of neurobiology. 57(3):303-322

Brustein, E., Marandi, N., Kovalchuk, Y., Drapeau, P., and Konnerth, A. (2003) "In vivo" monitoring of neuronal network activity in zebrafish by two-photon Ca(2+) imaging. Pflugers Archiv : European journal of physiology. 446(6):766-773

Buss, R.R., Bourque, C.W., and Drapeau, P. (2003) Membrane properties related to the firing behavior of zebrafish motoneurons. Journal of neurophysiology. 89(2):657-664

Saint-Amant, L. and Drapeau, P. (2003) Whole-cell patch-clamp recordings from identified spinal neurons in the zebrafish embryo. Methods in cell science : an official journal of the Society for In Vitro Biology. 25(1-2):59-64

Brustein, E., Saint-Amant, L., Buss, R.R., Chong, M., McDearmid, J.R., and Drapeau, P. (2003) Steps during the development of the zebrafish locomotor network. Journal of physiology, Paris. 97(1):77-86

Drapeau, P., Saint-Amant, L., Buss, R.R., Chong, M., McDearmid, J.R., and Brustein E. (2002) Development of the locomotor network in zebrafish. Prog. Neurobiol.. 68(2):85-111

Buss, R.R. and Drapeau, P. (2002) Activation of embryonic red and white muscle fibers during fictive swimming in the developing zebrafish. Journal of neurophysiology. 87:1244-1251

Brent, L. and Drapeau, P. (2002) Targeted 'knockdown' of channel expression in vivo with an antisense morpholino oligonucleotide. Neuroscience. 114(2):275-278

Drapeau, P., Buss, R.R., Ali, D.W., Legendre, P., and Rotundo, R.L. (2001) Limits to the development of fast neuromuscular transmission in zebrafish. Journal of neurophysiology. 86(6):2951-2956

Saint-Amant, L. and Drapeau, P. (2001) Synchronization of an embryonic network of identified spinal interneurons solely by electrical coupling. Neuron. 31(6):1035-1046

Buss, R.R. and Drapeau, P. (2001) Synaptic drive to motoneurons during fictive swimming in the developing zebrafish. Journal of neurophysiology. 86(1):197-210

Suwa, H., Saint-Amant, L., Triller, A., Drapeau, P., and Legendre, P. (2001) High-affinity zinc potentiation of inhibitory postsynaptic glycinergic currents in the zebrafish hindbrain. Journal of neurophysiology. 85(2):912-925

Drapeau, P. and Legendre, P. (2001) Neuromuscular transmission on the rebound. Receptors & channels. 7(6):491-496

Ali, D.W., Drapeau, P., and Legendre, P. (2000) Development of spontaneous glycinergic currents in the mauthner neuron of the zebrafish embryo. Journal of neurophysiology. 84(4):1726-1736

Buss, R.R. and Drapeau, P. (2000) Physiological properties of zebrafish embryonic red and white muscle fibers during early development. Journal of neurophysiology. 84(3):1545-1557

Saint-Amant, L. and Drapeau, P. (2000) Motoneuron activity patterns related to the earliest behavior of the zebrafish embryo. The Journal of neuroscience : the official journal of the Society for Neuroscience. 20(11):3964-3972

Legendre P., Ali, D.W., and Drapeau, P. (2000) Recovery from open channel block by acetylcholine during neuromuscular transmission in zebrafish. The Journal of neuroscience : the official journal of the Society for Neuroscience. 20(1):140-148

Ali, D.W., Buss, R.R., and Drapeau, P. (2000) Properties of miniature glutamatergic EPSCs in neurons of the locomotor regions of the developing zebrafish. Journal of neurophysiology. 83(1):181-191

Nguyen, P.V., Aniksztejn, L., Catarsi, S., and Drapeau, P. (1999) Maturation of neuromuscular transmission during early development in zebrafish. Journal of neurophysiology. 81(6):2852-2861

Drapeau, P., Ali, D.W., Buss, R.R., and Saint-Amant, L. (1999) In vivo recording from identifiable neurons of the locomotor network in the developing zebrafish. Journal of Neuroscience Methods. 88(1):1-13

Saint-Amant, L. and Drapeau, P. (1998) Time course of the development of motor behaviors in the zebrafish embryo. Journal of neurobiology. 37:622-632

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