PUBLICATION

A Novel Tool to Measure Extracellular Glutamate in the Zebrafish Nervous System In Vivo

Authors
MacDonald, R.B., Kashikar, N.D., Lagnado, L., Harris, W.A.
ID
ZDB-PUB-161228-3
Date
2017
Source
Zebrafish   14(3): 284-286 (Journal)
Registered Authors
Harris, William A., Lagnado, Leon, MacDonald, Ryan
Keywords
glia, glutamate, nervous system, retina
MeSH Terms
  • Glutamic Acid/metabolism*
  • Neuroglia/cytology
  • Neuroglia/metabolism
  • Zebrafish/growth & development
  • Zebrafish/metabolism*
  • Animals
  • Animals, Genetically Modified/growth & development
  • Animals, Genetically Modified/metabolism
  • Nervous System/cytology
  • Nervous System/metabolism*
  • Recombinant Fusion Proteins/metabolism*
  • Promoter Regions, Genetic
  • Gene Expression Regulation
  • Green Fluorescent Proteins/metabolism*
  • Retina/cytology
  • Retina/metabolism
  • Escherichia coli Proteins/metabolism*
(all 17)
PubMed
28027028 Full text @ Zebrafish
Abstract
Glutamate is the major excitatory neurotransmitter in the brain. Its release and eventual recycling are key to rapid sustained neural activity. We have paired the gfap promoter region with the glutamate reporter molecule, iGluSnFR, to drive expression in glial cells throughout the nervous system. Tg(gfap:iGluSnFR) is expressed on the glial membrane of Müller glia cells in the retina, which rapidly respond to stimulation and the release of extracellular glutamate. As glial cells are associated with most, if not all, synapses, Tg(gfap:iGluSnFR) is a novel and exciting tool to measure neuronal activity and extracellular glutamate dynamics in many regions of the nervous system.
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