PUBLICATION
Monitoring Toxic Ionic Liquids in Zebrafish (Danio rerio) with Desorption Electrospray Ionization Mass Spectrometry Imaging (DESI-MSI)
- Authors
- Perez, C.J., Tata, A., de Campos, M.L., Peng, C., Ifa, D.R.
- ID
- ZDB-PUB-161026-6
- Date
- 2017
- Source
- Journal of the American Society for Mass Spectrometry 28(6): 1136-1148 (Journal)
- Registered Authors
- Peng, Chun
- Keywords
- Desorption electrospray ionization, Environmental analysis, Imaging, Ionic liquids, Toxicology
- MeSH Terms
-
- Animals
- Biodegradation, Environmental
- Blood-Brain Barrier/drug effects
- Ecotoxicology/methods
- Environmental Monitoring/methods
- Gills/chemistry
- Gills/drug effects
- Ionic Liquids/analysis*
- Ionic Liquids/pharmacokinetics*
- Ionic Liquids/toxicity
- Spectrometry, Mass, Electrospray Ionization/methods*
- Tandem Mass Spectrometry
- Tissue Distribution
- Water Pollutants, Chemical/analysis
- Water Pollutants, Chemical/pharmacokinetics
- Water Pollutants, Chemical/toxicity
- Zebrafish*
- PubMed
- 27778241 Full text @ J. Am. Soc. Mass Spectrom.
Citation
Perez, C.J., Tata, A., de Campos, M.L., Peng, C., Ifa, D.R. (2017) Monitoring Toxic Ionic Liquids in Zebrafish (Danio rerio) with Desorption Electrospray Ionization Mass Spectrometry Imaging (DESI-MSI). Journal of the American Society for Mass Spectrometry. 28(6):1136-1148.
Abstract
Ambient mass spectrometry imaging has become an increasingly powerful technique for the direct analysis of biological tissues in the open environment with minimal sample preparation and fast analysis times. In this study, we introduce desorption electrospray ionization mass spectrometry imaging (DESI-MSI) as a novel, rapid, and sensitive approach to localize the accumulation of a mildly toxic ionic liquid (IL), AMMOENG 130 in zebrafish (Danio rerio). The work demonstrates that DESI-MSI has the potential to rapidly monitor the accumulation of IL pollutants in aquatic organisms. AMMOENG 130 is a quaternary ammonium-based IL reported to be broadly used as a surfactant in commercialized detergents. It is known to exhibit acute toxicity to zebrafish causing extensive damage to gill secondary lamellae and increasing membrane permeability. Zebrafish were exposed to the IL in a static 96-h exposure study in concentrations near the LC50 of 1.25, 2.5, and 5.0 mg/L. DESI-MS analysis of zebrafish gills demonstrated the appearance of a dealkylated AMMOENG 130 metabolite in the lowest concentration of exposure identified by a high resolution hybrid LTQ-Orbitrap mass spectrometer as the trimethylstearylammonium ion, [C21H46N]+. With DESI-MSI, the accumulation of AMMOENG 130 and its dealkylated metabolite in zebrafish tissue was found in the nervous and respiratory systems. AMMOENG 130 and the metabolite were capable of penetrating the blood brain barrier of the fish with significant accumulation in the brain. Hence, we report for the first time the simultaneous characterization, distribution, and metabolism of a toxic IL in whole body zebrafish analyzed by DESI-MSI. This ambient mass spectrometry imaging technique shows great promise for the direct analysis of biological tissues to qualitatively monitor foreign, toxic, and persistent compounds in aquatic organisms from the environment. Graphical Abstract ᅟ.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping