An alternative novel tool for DNA editing without target sequence limitation: the structure-guided nuclease
- Xu, S., Cao, S., Zou, B., Yue, Y., Gu, C., Chen, X., Wang, P., Dong, X., Xiang, Z., Li, K., Zhu, M., Zhao, Q., Zhou, G.
- Genome biology 17: 186 (Journal)
- Registered Authors
- Zhao, Qingshun
- DNA editing, Fok I, Guide DNA, Structure-guided endonuclease, flap endonuclease-1 (FEN-1)
- MeSH Terms
- Base Sequence
- DNA, Single-Stranded/genetics
- DNA, Single-Stranded/metabolism
- Deoxyribonucleases, Type II Site-Specific/chemistry
- Deoxyribonucleases, Type II Site-Specific/genetics
- Deoxyribonucleases, Type II Site-Specific/metabolism
- Flap Endonucleases/chemistry
- Flap Endonucleases/genetics
- Flap Endonucleases/metabolism
- Gene Editing*
- Gene Targeting/methods
- Models, Biological
- Sequence Analysis, DNA
- Substrate Specificity
- 27634179 Full text @ Genome Biol.
Xu, S., Cao, S., Zou, B., Yue, Y., Gu, C., Chen, X., Wang, P., Dong, X., Xiang, Z., Li, K., Zhu, M., Zhao, Q., Zhou, G. (2016) An alternative novel tool for DNA editing without target sequence limitation: the structure-guided nuclease. Genome biology. 17:186.
Engineered endonucleases are a powerful tool for editing DNA. However, sequence preferences may limit their application. We engineer a structure-guided endonuclease (SGN) composed of flap endonuclease-1 (FEN-1), which recognizes the 3' flap structure, and the cleavage domain of Fok I (Fn1), which cleaves DNA strands. The SGN recognizes the target DNA on the basis of the 3' flap structure formed between the target and the guide DNA (gDNA) and cut the target through its Fn1 dimerization. Our results show that the SGN, guided by a pair of gDNAs, cleaves transgenic reporter gene and endogenous genes in zebrafish embryonic genome.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes