PUBLICATION

Important role of endocannabinoid signaling in the development of functional vision and locomotion in zebrafish

Authors
Martella, A., Sepe, R.M., Silvestri, C., Zang, J., Fasano, G., Carnevali, O., De Girolamo, P., Neuhauss, S.C., Sordino, P., Di Marzo, V.
ID
ZDB-PUB-160915-7
Date
2016
Source
FASEB journal : official publication of the Federation of American Societies for Experimental Biology   30(12): 4275-4288 (Journal)
Registered Authors
Carnevali, Oliana, Neuhauss, Stephan, Sordino, Paolo, Zang, Jingjing
Keywords
2-arachidonoylglycerol, CB1, DAGL, MAGL, axonal pathfinding
MeSH Terms
  • Animals
  • Signal Transduction*/physiology
  • Endocannabinoids/metabolism*
  • Brain/metabolism*
  • Axons/metabolism*
  • Axons/ultrastructure
  • Locomotion/physiology*
  • Lipoprotein Lipase/metabolism*
  • Zebrafish
  • Behavior, Animal/physiology
(all 10)
PubMed
27623930 Full text @ FASEB J.
Abstract
The developmental role of the endocannabinoid system still remains to be fully understood. Here, we report the presence of a complete endocannabinoid system during zebrafish development and show that the genes that code for enzymes that catalyze the anabolism and catabolism (mgll and dagla) of the endocannabinoid, 2-AG (2-arachidonoylglycerol), as well as 2-AG main receptor in the brain, cannabinoid receptor type 1, are coexpressed in defined regions of axonal growth. By using morpholino-induced transient knockdown of the zebrafish Daglα homolog and its pharmacologic rescue, we suggest that synthesis of 2-AG is implicated in the control of axon formation in the midbrain-hindbrain region and that animals that lack Daglα display abnormal physiological behaviors in tests that measure stereotyped movement and motion perception. Our results suggest that the well-established role for 2-AG in axonal outgrowth has implications for the control of vision and movement in zebrafish and, thus, is likely common to all vertebrates.-Martella, A., Sepe, R. M., Silvestri, C., Zang, J., Fasano, G., Carnevali, O., De Girolamo, P., Neuhauss, S. C. F., Sordino, P., Di Marzo, V. Important role of endocannabinoid signaling in the development of functional vision and locomotion in zebrafish.
Genes / Markers
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Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
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Citation
Martella, A., Sepe, R.M., Silvestri, C., Zang, J., Fasano, G., Carnevali, O., De Girolamo, P., Neuhauss, S.C., Sordino, P., Di Marzo, V. (2016) Important role of endocannabinoid signaling in the development of functional vision and locomotion in zebrafish. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 30(12):4275-4288.