Melanoma Regression and Recurrence in Zebrafish
- Wojciechowska, S., Zeng, Z., Lister, J.A., Ceol, C.J., Patton, E.E.
- Methods in molecular biology (Clifton, N.J.) 1451: 143-53 (Chapter)
- Registered Authors
- Ceol, Craig, Lister, James A., Patton, E. Elizabeth, Zeng, Zhiqiang
- Melanoma, Mitf, Recurrence, Regression, Zebrafish cancer models
- MeSH Terms
- Microphthalmia-Associated Transcription Factor/genetics
- Microphthalmia-Associated Transcription Factor/metabolism
- Proto-Oncogene Proteins B-raf/genetics
- 27464806 Full text @ Meth. Mol. Biol.
Wojciechowska, S., Zeng, Z., Lister, J.A., Ceol, C.J., Patton, E.E. (2016) Melanoma Regression and Recurrence in Zebrafish. Methods in molecular biology (Clifton, N.J.). 1451:143-53.
Melanoma is the most lethal form of skin cancer with high mortality rates. Most melanoma cases have activating mutations in BRAF (V600E) and the selective inhibitors of BRAF(V600E) have been successfully used in patients. However, after initial tumor regression, the majority of patients develop drug resistance resulting in tumor regrowth. It is therefore important to understand the mechanisms underlying these processes. We have recently described the role of the master melanocyte transcription factor MITF in tumor growth, regression, and recurrence. Here, we describe protocols to study regression and recurrence in vivo, as well as for histology and immunohistochemistry, using a temperature-sensitive zebrafish model of human melanoma.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes