PUBLICATION

Establishment of a drug evaluation model against light-induced retinal degeneration using adult pigmented zebrafish

Authors

Saito, Y., Tsuruma, K., Shimazawa, M., Nishimura, Y., Tanaka, T., Hara, H.

ID

ZDB-PUB-160721-6

Date

2016

Source

Journal of Pharmacological Sciences 131(3): 215-8 (Journal)

Registered Authors

Tanaka, Toshio

Keywords

Dry age-related macular degeneration, Pigmented retina, Zebrafish

MeSH Terms

Animals
Light*
Administration, Ophthalmic
Macular Degeneration/drug therapy*
Macular Degeneration/etiology
Zebrafish
Disease Models, Animal*
Vitreous Body
Acetylcysteine/administration & dosage
Acetylcysteine/therapeutic use*

(all 10)

PubMed

27430989 Full text @ J. Pharmacol. Sci.

Abstract

Age-related macular degeneration (AMD) is a major cause of irreversible loss of central vision in the elderly. Zebrafish is an attractive animal model in some respects, lower cost, smaller housing facilities and easier genetic manipulation compared to rodents. The present study aimed to establish a drug evaluation method against light irradiation, as a dry AMD disease model, using adult pigmented zebrafish. Intravitreal administration of an antioxidant, N-acetylcysteine, protected against light-induced retinal degeneration in a concentration-dependent manner. We established a new drug evaluation model against light-induced retinal degeneration that can provide new knowledge about dry AMD pathology and therapy.

Genes / Markers

Figures

Show all Figures

Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT	constant light	Adult	retinal outer nuclear layer cell death increased occurrence, abnormal	Fig. 3
WT	constant light	Adult	retinal outer nuclear layer decreased thickness, abnormal	Fig. 1
WT	constant light	Adult	retinal outer nuclear layer decreased thickness, abnormal	Fig. 2
WT	constant light	Adult	retinal outer nuclear layer photoreceptor cell decreased amount, abnormal	Fig. 1
WT	constant light	Adult	retinal outer nuclear layer photoreceptor cell decreased amount, abnormal	Fig. 2
WT	constant light, chemical treatment by injection: N-acetyl-L-cysteine	Adult	retinal outer nuclear layer cell death increased occurrence, ameliorated	Fig. 3
WT	constant light, chemical treatment by injection: N-acetyl-L-cysteine	Adult	retinal outer nuclear layer photoreceptor cell amount, ameliorated	Fig. 2
WT	constant light, chemical treatment by injection: N-acetyl-L-cysteine	Adult	retinal outer nuclear layer thickness, ameliorated	Fig. 2

1 - 8 of 8

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Mutations / Transgenics

Human Disease / Model

Human Disease	Fish	Conditions	Evidence
macular degeneration	WT	constant light	TAS

Sequence Targeting Reagents

Fish

Fish
WT

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Saito et al., 2016 ZDB-PUB-160721-6 PMID:27430989

Establishment of a drug evaluation model against light-induced retinal degeneration using adult pigmented zebrafish

Saito et al., 2016

ZDB-PUB-160721-6
PMID:27430989