PUBLICATION
Differences in Strength and Timing of the mtDNA Bottleneck between Zebrafish Germline and Non-germline Cells
- Authors
- Otten, A.B., Theunissen, T.E., Derhaag, J.G., Lambrichs, E.H., Boesten, I.B., Winandy, M., van Montfoort, A.P., Tarbashevich, K., Raz, E., Gerards, M., Vanoevelen, J.M., van den Bosch, B.J., Muller, M., Smeets, H.J.
- ID
- ZDB-PUB-160706-14
- Date
- 2016
- Source
- Cell Reports 16(3): 622-30 (Journal)
- Registered Authors
- Muller, Marc, Raz, Erez, Tarbashevich, Katsiyarina, Winandy, Marie
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Differentiation/genetics
- DNA Replication/genetics
- DNA, Mitochondrial/genetics*
- Embryonic Development/genetics
- Female
- Gene Dosage/genetics
- Germ Cells/metabolism*
- Mitochondria/genetics
- Mutation/genetics
- Oocytes/metabolism
- Oogenesis/genetics
- Zebrafish/genetics*
- PubMed
- 27373161 Full text @ Cell Rep.
Citation
Otten, A.B., Theunissen, T.E., Derhaag, J.G., Lambrichs, E.H., Boesten, I.B., Winandy, M., van Montfoort, A.P., Tarbashevich, K., Raz, E., Gerards, M., Vanoevelen, J.M., van den Bosch, B.J., Muller, M., Smeets, H.J. (2016) Differences in Strength and Timing of the mtDNA Bottleneck between Zebrafish Germline and Non-germline Cells. Cell Reports. 16(3):622-30.
Abstract
We studied the mtDNA bottleneck in zebrafish to elucidate size, timing, and variation in germline and non-germline cells. Mature zebrafish oocytes contain, on average, 19.0 × 10(6) mtDNA molecules with high variation between oocytes. During embryogenesis, the mtDNA copy number decreases to ∼170 mtDNA molecules per primordial germ cell (PGC), a number similar to that in mammals, and to ∼50 per non-PGC. These occur at the same developmental stage, implying considerable variation in mtDNA copy number in (non-)PGCs of the same female, dictated by variation in the mature oocyte. The presence of oocytes with low mtDNA numbers, if similar in humans, could explain how (de novo) mutations can reach high mutation loads within a single generation. High mtDNA copy numbers in mature oocytes are established by mtDNA replication during oocyte development. Bottleneck differences between germline and non-germline cells, due to early differentiation of PGCs, may account for different distribution patterns of familial mutations.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping