PUBLICATION
Eph-ephrin signaling maintains the boundary of dorsal forerunner cell cluster during morphogenesis of the zebrafish embryonic left-right organizer
- Authors
- Zhang, J.F., Jiang, Z., Liu, X., Meng, A.
- ID
- ZDB-PUB-160612-7
- Date
- 2016
- Source
- Development (Cambridge, England) 143(14): 2603-15 (Journal)
- Registered Authors
- Meng, Anming, Zhang, Junfeng
- Keywords
- Eph, Ephrin, Left-right, Asymmetry, DFCs, Kupffer's vesicle, Embryo, Zebrafish
- MeSH Terms
-
- Functional Laterality
- Zebrafish Proteins/metabolism*
- Mesoderm/cytology
- Cell Communication
- Embryo, Nonmammalian/cytology*
- Embryo, Nonmammalian/metabolism
- Cell Movement
- Zebrafish/embryology*
- Body Patterning*
- Organizers, Embryonic/cytology*
- Organizers, Embryonic/metabolism
- Cell Aggregation
- Gene Knockout Techniques
- Ephrins/metabolism*
- Animals
- rho GTP-Binding Proteins/metabolism
- Signal Transduction*
- Morphogenesis*
- Receptor, EphB4/metabolism*
- Mutation/genetics
- PubMed
- 27287807 Full text @ Development
Citation
Zhang, J.F., Jiang, Z., Liu, X., Meng, A. (2016) Eph-ephrin signaling maintains the boundary of dorsal forerunner cell cluster during morphogenesis of the zebrafish embryonic left-right organizer. Development (Cambridge, England). 143(14):2603-15.
Abstract
The kupffer's vesicle (KV) is the so-called left-right organizer in teleost fishes. KV is formed from dorsal forerunner cells (DFCs) and generates asymmetrical signals for breaking symmetry of embryos. It is unclear how DFCs or KV cells are prevented from intermingling with adjacent cells. In this study, we show that the Eph receptor gene ephb4b is highly expressed in DFCs while the ephrin ligand genes including efnb2b are expressed in cells next to the DFCs cluster during zebrafish gastrulation. ephb4b knockdown or mutation and efnb2b knockdown cause dispersal of DFCs, a smaller KV and randomization of laterality organs. The DFCs often dynamically form lamellipodium-like, bleb-like and filopodium-like membrane protrusions at the interface, which attempt to invade but are bounced back by adjacent non-DFC cells during gastrulation. Upon inhibition of Eph-ephrin signaling, however, the repulsion between DFCs and non-DFC cells is weakened or lost, allowing DFCs to migrate away. Ephb4b/efnb2b signaling by activating RhoA activity mediates contact and repulsion between DFCs and neighboring cells during gastrulation, preventing intermingling of different cell populations. Therefore, our data uncover an important role of Eph/ephrin signaling in maintaining DFCs boundary and KV boundary for normal left-right asymmetrical development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping