PUBLICATION
TBK1-like transcript negatively regulates the production of IFN and IFN-stimulated genes through RLRs-MAVS-TBK1 pathway
- Authors
- Zhang, L., Chen, W.Q., Hu, Y.W., Wu, X.M., Nie, P., Chang, M.X.
- ID
- ZDB-PUB-160411-4
- Date
- 2016
- Source
- Fish & shellfish immunology 54: 135-43 (Journal)
- Registered Authors
- Chang, Mingxian, Nie, Pin
- Keywords
- IFN, Innate immune signaling, RIG-I-like receptors, TBK1, TBK1-like transcript, zebrafish
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Fish Diseases/immunology*
- Fish Diseases/virology
- Immunity, Innate*
- Interferons/genetics*
- Interferons/metabolism
- Phylogeny
- Protein Serine-Threonine Kinases/chemistry
- Protein Serine-Threonine Kinases/genetics*
- Protein Serine-Threonine Kinases/metabolism
- Rhabdoviridae/physiology*
- Rhabdoviridae Infections/immunology
- Rhabdoviridae Infections/veterinary*
- Rhabdoviridae Infections/virology
- Signal Transduction
- Zebrafish*
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 27060200 Full text @ Fish Shellfish Immunol.
Citation
Zhang, L., Chen, W.Q., Hu, Y.W., Wu, X.M., Nie, P., Chang, M.X. (2016) TBK1-like transcript negatively regulates the production of IFN and IFN-stimulated genes through RLRs-MAVS-TBK1 pathway. Fish & shellfish immunology. 54:135-43.
Abstract
TANK-binding kinase 1 (TBK1) is an essential serine/threonine-protein kinase required for Toll-like receptor (TLR)- and retinoic acid-inducible gene I (RIG-I) -mediated induction of type I IFN and host antiviral defense. In the present study, TBK1-like transcript, namely TBK1L, was cloned from zebrafish. Compared with TBK1, TBK1L contains an incomplete S_TKc domain, and lacks UBL_TBK1_like domain. Realtime PCR showed that TBK1L was constitutively produced in embryos, early larvae and ZF4 cells, and unchanged in ZF4 cells following SVCV infection. Overexpression of TBK1 but not TBK1L resulted in significant activation of zebrafish IFN1 and IFN3 promoters. Similarily, TBK1L had little impact on the antiviral state of the cells. However, the overexpression of TBK1L negatively regulated the induction of zebrafish IFN1 and/or IFN3 promoters mediated by the retinoic acid-inducible gene I-like receptors (RLRs), MAVS and TBK1. In addition, the overexpression of TBK1L in zebrafish embryos led to the decreased production of many IFN-stimulated genes induced by TBK1. Collectively, these data support that zebrafish TBK1L negatively regulates RLRs-MAVS-TBK1 pathway.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping