PUBLICATION
In Vivo Ca(2+) Imaging Reveals that Decreased Dendritic Excitability Drives Startle Habituation
- Authors
- Marsden, K.C., Granato, M.
- ID
- ZDB-PUB-151216-28
- Date
- 2015
- Source
- Cell Reports 13: 1733-40 (Journal)
- Registered Authors
- Granato, Michael, Marsden, Kurt
- Keywords
- none
- MeSH Terms
-
- Animals
- Calcium/metabolism*
- Calcium Signaling/drug effects
- Dendrites/metabolism*
- Dizocilpine Maleate/pharmacology
- Larva/metabolism
- Neuronal Calcium-Sensor Proteins/metabolism
- Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors
- Receptors, N-Methyl-D-Aspartate/metabolism
- Reflex, Startle/drug effects
- Strychnine/pharmacology
- Zebrafish/growth & development
- Zebrafish/metabolism
- PubMed
- 26655893 Full text @ Cell Rep.
Citation
Marsden, K.C., Granato, M. (2015) In Vivo Ca(2+) Imaging Reveals that Decreased Dendritic Excitability Drives Startle Habituation. Cell Reports. 13:1733-40.
Abstract
Exposure to repetitive startling stimuli induces habitation, a simple form of learning. Despite its simplicity, the precise cellular mechanisms by which repeated stimulation converts a robust behavioral response to behavioral indifference are unclear. Here, we use head-restrained zebrafish larvae to monitor subcellular Ca(2+) dynamics in Mauthner neurons, the startle command neurons, during startle habituation in vivo. Using the Ca(2+) reporter GCaMP6s, we find that the amplitude of Ca(2+) signals in the lateral dendrite of the Mauthner neuron determines startle probability and that depression of this dendritic activity rather than downstream inhibition mediates glycine and N-methyl-D-aspartate (NMDA)-receptor-dependent short-term habituation. Combined, our results suggest a model for habituation learning in which increased inhibitory drive from feedforward inhibitory neurons combined with decreased excitatory input from auditory afferents decreases dendritic and Mauthner neuron excitability.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping