PUBLICATION

TRP channel mediated neuronal activation and ablation in freely behaving zebrafish

Authors
Chen, S., Chiu, C.N., McArthur, K.L., Fetcho, J.R., Prober, D.A.
ID
ZDB-PUB-151216-20
Date
2016
Source
Nature Methods   13(2): 147-50 (Journal)
Registered Authors
Fetcho, Joseph R., Prober, David
Keywords
Behavioural methods, Genetic models, Neuroscience, Patch clamp, Zebrafish
MeSH Terms
  • Animals
  • Embryo, Nonmammalian
  • Gene Expression Regulation/physiology*
  • Sensory Receptor Cells/physiology*
  • Transient Receptor Potential Channels/physiology*
  • Zebrafish
PubMed
26657556 Full text @ Nat. Methods
Abstract
The zebrafish (Danio rerio) is a useful vertebrate model system in which to study neural circuits and behavior, but tools to modulate neurons in freely behaving animals are limited. As poikilotherms that live in water, zebrafish are amenable to thermal and pharmacological perturbations. We exploit these properties by using transient receptor potential (TRP) channels to activate or ablate specific neuronal populations using the chemical and thermal agonists of heterologously expressed TRPV1, TRPM8 and TRPA1.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes