PUBLICATION
Identification and Expression Analysis of Zebrafish (Danio rerio) E-Selectin during Embryonic Development
- Authors
- Sun, G., Liu, K., Wang, X., Liu, X., He, Q., Hsiao, C.D.
- ID
- ZDB-PUB-151019-9
- Date
- 2015
- Source
- Molecules 20: 18539-18550 (Journal)
- Registered Authors
- Hsiao, Chung-Der
- Keywords
- E-selectin, expression, identification, lipopolysaccharide, zebrafish
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cloning, Molecular
- Conserved Sequence
- E-Selectin/genetics
- E-Selectin/immunology
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/immunology
- Embryo, Nonmammalian/metabolism
- Embryonic Development
- Endothelial Cells/cytology
- Endothelial Cells/drug effects
- Endothelial Cells/immunology
- Gene Expression Regulation, Developmental*
- Hematopoiesis/drug effects
- Hematopoiesis/genetics*
- Hematopoiesis/immunology
- Humans
- Inflammation
- Interleukin-1beta/genetics
- Interleukin-1beta/immunology
- Lipopolysaccharides/pharmacology
- Mice
- Molecular Sequence Data
- Neovascularization, Physiologic/drug effects
- Neovascularization, Physiologic/genetics*
- Neovascularization, Physiologic/immunology
- Open Reading Frames
- Phylogeny
- Sequence Alignment
- Stem Cells/cytology
- Stem Cells/drug effects
- Stem Cells/immunology
- Tumor Necrosis Factor-alpha/genetics
- Tumor Necrosis Factor-alpha/immunology
- Zebrafish/classification
- Zebrafish/genetics*
- Zebrafish/growth & development
- Zebrafish/immunology
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/immunology
- PubMed
- 26473817 Full text @ Molecules
Citation
Sun, G., Liu, K., Wang, X., Liu, X., He, Q., Hsiao, C.D. (2015) Identification and Expression Analysis of Zebrafish (Danio rerio) E-Selectin during Embryonic Development. Molecules. 20:18539-18550.
Abstract
In this study, we cloned the full-length cDNA of E-selectin of zebrafish (Danio rerio), analyzed its expression pattern and preliminarily explored its biological function. Zebrafish E-selectin cDNA is 3146 bp and encodes a putative 871 amino acid protein. All structural domains involved in E-selectin function are conserved in the putative protein. Whole-mount in situ hybridization of zebrafish at 24 and 48 h post-fertilization (hpf) revealed E-selectin expression mainly in vascular/endothelial progenitor cells in the posterior trunk and blood cells in the intermediate cell mass and posterior cardinal vein regions. Real-time quantitative RT-PCR analysis detected E-selectin expression at 0.2, 24 and 48 hpf and significantly decreased from 48 to 72 hpf. The expression of E-selectin, tumor necrosis factor-α and interleukin-1β was significantly upregulated at 22 to 72 h after induction with bacterial lipopolysaccharide. Thus, the structure of E-selectin protein is highly conserved among species, and E-selectin may be involved in embryonic development and essential for hematopoiesis and angiogenesis during embryonic development in zebrafish. Furthermore, we provide the first evidence of inflammatory mediators inducing E-selectin expression in non-mammalian vertebrates, which suggests that zebrafish E-selectin may be involved in inflammation and probably has similar biological function to mammalian E-selectin.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping