ZFIN ID: ZDB-PUB-151011-3
Parallelism and epistasis in skeletal evolution identified through use of phylogenomic mapping strategies
Daane, J.M., Rohner, N., Konstantinidis, P., Djuranovic, S., Harris, M.P.
Date: 2016
Source: Molecular Biology and Evolution   33(1): 162-73 (Journal)
Registered Authors: Harris, Matthew, Rohner, Nicolas
Keywords: comparative genomics, nonmodel organisms, parallelism, zebrafish, fgf signaling, epistasis
MeSH Terms:
  • Animals
  • Biological Evolution*
  • Bone and Bones/physiology*
  • Chromosome Mapping/methods*
  • Epistasis, Genetic/genetics*
  • Genomics/methods*
  • Phylogeny
  • Zebrafish/genetics
PubMed: 26452532 Full text @ Mol Bio Evol
The identification of genetic mechanisms underlying evolutionary change is critical to our understanding of natural diversity, but is presently limited by the lack of genetic and genomic resources for most species. Here, we present a new comparative genomic approach that can be applied to a broad taxonomic sampling of non-model species to investigate the genetic basis of evolutionary change. Using our analysis pipeline, we show that duplication and divergence of fgfr1a is correlated with the reduction of scales within fishes of the genus Phoxinellus. As a parallel genetic mechanism is observed in scale-reduction within independent lineages of cypriniforms, our finding exposes significant developmental constraint guiding morphological evolution. We further identified fixed variation in fgf20a within Phoxinellus and demonstrate that combinatorial loss-of-function of fgfr1a and fgf20a within zebrafish phenocopies the evolved scalation pattern. Together, these findings reveal epistatic interactions between fgfr1a and fgf20a as a developmental mechanism regulating skeletal variation amongst fishes.