PUBLICATION
Copper Oxide Nanoparticles Reduce Vasculogenesis in Transgenic Zebrafish Through Down-Regulation of Vascular Endothelial Growth Factor Expression and Induction of Apoptosis
- Authors
- Chang, J., Ichihara, G., Shimada, Y., Tada-Oikawa, S., Kuroyanagi, J., Zhang, B., Suzuki, Y., Sehsah, R., Kato, M., Tanaka, T., Ichihara, S.
- ID
- ZDB-PUB-150929-7
- Date
- 2015
- Source
- Journal of nanoscience and nanotechnology 15: 2140-7 (Journal)
- Registered Authors
- Tanaka, Toshio
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Apoptosis/drug effects*
- Cell Survival/drug effects
- Copper/chemistry
- Copper/pharmacology*
- Down-Regulation/drug effects*
- Human Umbilical Vein Endothelial Cells/cytology
- Human Umbilical Vein Endothelial Cells/drug effects
- Humans
- Nanoparticles*
- Neovascularization, Physiologic/drug effects*
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Vascular Endothelial Growth Factor A/genetics*
- Zebrafish*
- PubMed
- 26413632 Full text @ J. Nanosci. Nanotechnol.
Citation
Chang, J., Ichihara, G., Shimada, Y., Tada-Oikawa, S., Kuroyanagi, J., Zhang, B., Suzuki, Y., Sehsah, R., Kato, M., Tanaka, T., Ichihara, S. (2015) Copper Oxide Nanoparticles Reduce Vasculogenesis in Transgenic Zebrafish Through Down-Regulation of Vascular Endothelial Growth Factor Expression and Induction of Apoptosis. Journal of nanoscience and nanotechnology. 15:2140-7.
Abstract
The present study investigated the effects of exposure to metal oxide nanoparticles on vasculogenesis/angiogenesis using transgenic zebrafish. The study also examined the potential mechanisms involved in those effects using human umbilical vein endothelial cells (HUVEC). TG (nacre/fli1:EGFP) zebrafish were exposed to nano-sized titanium dioxide (TiO2), silica dioxide (SiO2), and copper oxide (CuO) particles at 0.01, 1 and 100 µg/ml concentrations from 1 to 5 dpf (day-post-fertilization). Angiogenesis was evaluated morphologically at the end of exposure. Exposure to CuO nanoparticles reduced the number of transversely-running subintestinal vessels in TG zebrafish. Exposure to CuO nanoparticles down-regulated the expression of vascular endothelial growth factor (VEGF) and VEGF receptor in endothelial cells sorted by Fluorescence Activated Cell Sorter (FACS). Exposure of HUVEC to CuO nanoparticles reduced cell viability and increased apoptotic index in a dose-dependent manner. The results suggested that CuO nanoparticles inhibit vasculogenesis through reduction of VEGF expression and induction of apoptosis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping