PUBLICATION
Repeated Blood Collection for Blood Tests in Adult Zebrafish
- Authors
- Zang, L., Shimada, Y., Nishimura, Y., Tanaka, T., Nishimura, N.
- ID
- ZDB-PUB-150919-3
- Date
- 2015
- Source
- Journal of visualized experiments : JoVE (102): e53272 (Journal)
- Registered Authors
- Tanaka, Toshio
- Keywords
- Repeated blood collection, adult zebrafish, dorsal aorta, hemoglobin, fasting blood glucose, plasma triacylglycerol, total cholesterol, animal model
- MeSH Terms
-
- Animals
- Blood Glucose/metabolism
- Blood Specimen Collection/methods
- Blood Specimen Collection/veterinary*
- Body Weight
- Disease Models, Animal
- Female
- Lipid Metabolism
- Male
- Obesity/blood
- Triglycerides/blood
- Zebrafish/blood*
- PubMed
- 26383512 Full text @ J. Vis. Exp.
Citation
Zang, L., Shimada, Y., Nishimura, Y., Tanaka, T., Nishimura, N. (2015) Repeated Blood Collection for Blood Tests in Adult Zebrafish. Journal of visualized experiments : JoVE. (102):e53272.
Abstract
Repeated blood collection is one of the most common techniques performed on laboratory animals. However, a non-lethal protocol for blood collection from zebrafish has not been established. The previous methods for blood collection from zebrafish are lethal, such as lateral incision, decapitation and tail ablation. Thus we have developed a novel "repeated" blood collection method, and present here a detailed protocol outlining this procedure. This method is minimally invasive and results in a very low mortality rate (2.3%) for zebrafish, thus enabling repeated blood sampling from the same individual. The maximum volume of blood sampling is dependent on body weight of the fish. The volume for repeated blood sampling at intervals should be ≤0.4% of body weight every week or ≤1% every 2 weeks, which were evaluated by measurements of blood hemoglobin. Additionally, hemoglobin, fasting blood glucose, plasma triacylglycerol (TG) and total cholesterol levels in male and female adult zebrafish were measured. We also applied this method to investigate the dysregulation of glucose metabolism in diet-induced obesity. This blood collection method will allow many applications, including glucose and lipid metabolism and hematological studies, which will increase the use of zebrafish as a human disease model organism.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping