ZFIN ID: ZDB-PUB-150711-11
Identification of polarized macrophage subsets in zebrafish
Nguyen Chi, M., Laplace-Builhe, B., Travnickova, J., Luz-Crawford, P., Tejedor, G., Phan, Q.T., Duroux-Richard, I., Levraud, J.P., Kissa, K., Lutfalla, G., Jorgensen, C., Djouad, F.
Date: 2015
Source: eLIFE   4: e07288 (Journal)
Registered Authors: Lutfalla, Georges, Phan, Quang Tien
Keywords: developmental biology, immunology, live imaging, macrophages, stem cells, zebrafish
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Escherichia coli Infections/immunology
  • Flow Cytometry
  • Gene Expression Profiling
  • Genes, Reporter
  • Macrophages/classification*
  • Macrophages/immunology*
  • Microscopy, Confocal
  • Molecular Sequence Data
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Tumor Necrosis Factor-alpha/biosynthesis
  • Wounds and Injuries/immunology
  • Zebrafish/immunology*
PubMed: 26154973 Full text @ Elife
While the mammalian macrophage phenotypes have been intensively studied in vitro, the dynamic of their phenotypic polarization has never been investigated in live vertebrates. We used the zebrafish as a live model to identify and trail macrophage subtypes. We generated a transgenic line whose macrophages expressing tnfa, a key feature of classically activated (M1) macrophages, express fluorescent proteins Tg(mpeg1:mCherryF/tnfa:eGFP-F). Using 4D-confocal microscopy, we showed that both aseptic wounding and E. coli inoculation triggered macrophage recruitment, some of which started to express tnfa. RT-qPCR on FACS-sorted tnfa+ and tnfa- macrophages showed that they respectively expressed M1 and alternatively activated (M2) mammalian markers. Fate tracing of tnfa+ macrophages during the time-course of inflammation demonstrated that pro-inflammatory macrophages converted into M2-like phenotype during the resolution step. Our results reveal the diversity and plasticity of zebrafish macrophage subsets and underline the similarities with mammalian macrophages proposing a new system to study macrophage functional dynamic.