PUBLICATION

A functional Bucky ball-GFP transgene visualizes germ plasm in living zebrafish

Authors
Riemer, S., Bontems, F., Krishnakumar, P., Gömann, J., Dosch, R.
ID
ZDB-PUB-150706-3
Date
2015
Source
Gene expression patterns : GEP   18(1-2): 44-52 (Journal)
Registered Authors
Bontems, Franck, Dosch, Roland, Krishnakumar, Pritesh, Riemer, Stephan
Keywords
Balbiani body, Bucky ball, Vasa, germline development, primordial germ cell, transgene
MeSH Terms
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/immunology
  • Zebrafish Proteins/metabolism
  • Animals, Genetically Modified
  • Green Fluorescent Proteins/genetics*
  • Antibodies/metabolism
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Larva/genetics
  • Larva/growth & development
  • Larva/metabolism
  • Animals
  • Germ Cells/metabolism*
  • Oogenesis
  • Recombinant Fusion Proteins/genetics
  • Oocytes/cytology
  • Oocytes/metabolism
(all 18)
PubMed
26143227 Full text @ Gene Expr. Patterns
Abstract
In many animals, the germline is specified by maternal RNA-granules termed germ plasm. The correct localization of germ plasm during embryogenesis is therefore crucial for the specification of germ cells. In zebrafish, we previously identified Bucky ball (Buc) as a key regulator of germ plasm formation. Here, we used a Buc antibody to describe its continuous germ plasm localization. Moreover, we generated a transgenic Buc-GFP line for live imaging, which visualizes germ plasm from its assembly during oogenesis up to the larval stages. Live imaging of Buc-GFP generated stunning movies, as they highlighted the dynamic details of germ plasm movements. Moreover, we discovered that Buc was still detected in primordial germ cells two days after fertilization. Interestingly, the transgene rescued buc mutants demonstrating genetically that the Buc-GFP fusion protein is functional. These results show that Buc-GFP exerts all biochemical interactions essential for germline development and highlight the potential of this line to analyze the molecular regulation of germ plasm formation.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
bucp106re/p106restandard conditionsAdult
bucp43bmtb/p43bmtbstandard conditionsAdult
1 - 2 of 2
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
a9
    		Complex
    ge2TgTransgenic Insertion
      p43bmtb
        		Point Mutation
          p106re
            		Point Mutation
            w2
              		Point Mutation
              1 - 5 of 5
              Show
              Human Disease / Model
              No data available
              Sequence Targeting Reagents
              No data available
              Fish
              Fish
              bucp43bmtb/p43bmtb
              bucp106re/p106re
              ge2Tg
              WT
              1 - 4 of 4
              Show
              Antibodies
              Name Type Antigen Genes Isotypes Host Organism
              Ab1-ddx4Rabbit
              Ab2-bucpolyclonalGuinea pig
              1 - 2 of 2
              Show
              Orthology
              No data available
              Engineered Foreign Genes
              Marker Marker Type Name
              EGFPEFGEGFP
              1 - 1 of 1
              Show
              Mapping
              No data available
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              Citation
              Riemer, S., Bontems, F., Krishnakumar, P., Gömann, J., Dosch, R. (2015) A functional Bucky ball-GFP transgene visualizes germ plasm in living zebrafish. Gene expression patterns : GEP. 18(1-2):44-52.