PUBLICATION
Comparative Effects of Mercury(II) and Cadmium on MutS Homolog 6(MSH6)-Mediated DNA Mismatch Binding Activities in Zebrafish (Danio rerio) Embryos
- Authors
- Ho, T.N., Sung, S.T., Huang, K.M., Hsu, T.
- ID
- ZDB-PUB-150702-7
- Date
- 2015
- Source
- Journal of biochemical and molecular toxicology 29(11): 513-20 (Journal)
- Registered Authors
- Hsu, Todd
- Keywords
- Cadmium, Mercury, Mismatch Repair, MutS homologs, Zebrafish
- MeSH Terms
-
- Animals
- Base Pair Mismatch*
- Cadmium/toxicity*
- DNA-Binding Proteins/metabolism*
- Mercury/toxicity*
- Oxidative Stress
- Protein Binding
- Zebrafish/embryology*
- PubMed
- 26130599 Full text @ J. Biochem. Mol. Toxicol.
- CTD
- 26130599
Citation
Ho, T.N., Sung, S.T., Huang, K.M., Hsu, T. (2015) Comparative Effects of Mercury(II) and Cadmium on MutS Homolog 6(MSH6)-Mediated DNA Mismatch Binding Activities in Zebrafish (Danio rerio) Embryos. Journal of biochemical and molecular toxicology. 29(11):513-20.
Abstract
MutS homolog 6 (MSH6) of the MSH2-MSH6 complex binds simple mispairs and small insertion-deletion loops (IDL) then initiates DNA mismatch repair in eukaryotes. We have shown the ability of Cd(2+) to downregulate msh2/msh6 expression in zebrafish embryos via oxidative stress. This study explored the effects of Cd(2+) and Hg(2+) on MSH6-mediated mismatch binding activities. MSH6-mediated G-T and IDL-specific binding activities were significantly inhibited at similar levels after exposing zebrafish embryos at 1 h postfertilization) to HgCl2 or CdCl2 at 1.0 to 2.5 μM for 9 h, but MSH6 synthesis was found to be less sensitive to Hg(2+) than to Cd(2+) . Real-time RT-PCR and in situ hybridization also detected a weaker susceptibility of MSH gene transcription to Hg(2+) . The weaker response of MSH gene activities to Hg(2+) correlated with the lower oxidative stress-inducing potential of Hg(2+) . Hence, Hg(2+) targets mismatch sensing capacity at protein function rather than at transcription level.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping