PUBLICATION
Cross-Scale Integrin Regulation Organizes ECM and Tissue Topology
- Authors
- Jülich, D., Cobb, G., Melo, A.M., McMillen, P., Lawton, A.K., Mochrie, S.G., Rhoades, E., Holley, S.A.
- ID
- ZDB-PUB-150623-8
- Date
- 2015
- Source
- Developmental Cell 34(1): 33-44 (Journal)
- Registered Authors
- Holley, Scott, Jülich, Dörthe, Lawton, Angela
- Keywords
- none
- MeSH Terms
-
- Animals
- Cadherins/metabolism
- Cell Adhesion/physiology
- Cell Membrane/metabolism
- Extracellular Matrix/metabolism*
- Extracellular Matrix Proteins/metabolism*
- Fibronectins/metabolism*
- Integrins/metabolism*
- Somites/metabolism*
- Zebrafish
- Zebrafish Proteins/metabolism
- PubMed
- 26096733 Full text @ Dev. Cell
Citation
Jülich, D., Cobb, G., Melo, A.M., McMillen, P., Lawton, A.K., Mochrie, S.G., Rhoades, E., Holley, S.A. (2015) Cross-Scale Integrin Regulation Organizes ECM and Tissue Topology. Developmental Cell. 34(1):33-44.
Abstract
The diverse morphologies of animal tissues are underlain by different configurations of adherent cells and extracellular matrix (ECM). Here, we elucidate a cross-scale mechanism for tissue assembly and ECM remodeling involving Cadherin 2, the ECM protein Fibronectin, and its receptor Integrin α5. Fluorescence cross-correlation spectroscopy within the zebrafish paraxial mesoderm mesenchyme reveals a physical association between Integrin α5 on adjacent cell membranes. This Integrin-Integrin complex correlates with conformationally inactive Integrin. Cadherin 2 stabilizes both the Integrin association and inactive Integrin conformation. Thus, Integrin repression within the adherent mesenchymal interior of the tissue biases Fibronectin fibrillogenesis to the tissue surface lacking cell-cell adhesions. Along nascent somite boundaries, Cadherin 2 levels decrease, becoming anti-correlated with levels of Integrin α5. Simultaneously, Integrin α5 clusters and adopts the active conformation and then commences ECM assembly. This cross-scale regulation of Integrin activation organizes a stereotypic pattern of ECM necessary for vertebrate body elongation and segmentation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping