PUBLICATION
Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration
- Authors
- Choi, T.Y., Khaliq, M., Ko, S., So, J., Shin, D.
- ID
- ZDB-PUB-150613-5
- Date
- 2015
- Source
- Journal of visualized experiments : JoVE (99): e52785 (Journal)
- Registered Authors
- Khaliq, Mehwish, Ko, Sungjin, Shin, Donghun, So, Juhoon
- Keywords
- none
- MeSH Terms
-
- Ablation Techniques/methods*
- Animals
- Animals, Genetically Modified
- Biliary Tract/cytology
- Biliary Tract/physiology*
- Female
- Hepatocytes/cytology*
- Hepatocytes/drug effects
- Liver/cytology
- Liver/drug effects
- Liver/physiology
- Liver Regeneration/physiology*
- Male
- Metronidazole/pharmacology
- Models, Animal
- Nitroreductases/biosynthesis
- Nitroreductases/genetics
- Zebrafish
- PubMed
- 26065829 Full text @ J. Vis. Exp.
Citation
Choi, T.Y., Khaliq, M., Ko, S., So, J., Shin, D. (2015) Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration. Journal of visualized experiments : JoVE. (99):e52785.
Abstract
The liver has a great capacity to regenerate. Hepatocytes, the parenchymal cells of the liver, can regenerate in one of two ways: hepatocyte- or biliary-driven liver regeneration. In hepatocyte-driven liver regeneration, regenerating hepatocytes are derived from preexisting hepatocytes, whereas, in biliary-driven regeneration, regenerating hepatocytes are derived from biliary epithelial cells (BECs). For hepatocyte-driven liver regeneration, there are excellent rodent models that have significantly contributed to the current understanding of liver regeneration. However, no such rodent model exists for biliary-driven liver regeneration. We recently reported on a zebrafish liver injury model in which BECs extensively give rise to hepatocytes upon severe hepatocyte loss. In this model, hepatocytes are specifically ablated by a pharmacogenetic means. Here we present in detail the methods to ablate hepatocytes and to analyze the BEC-driven liver regeneration process. This hepatocyte-specific ablation model can be further used to discover the underlying molecular and cellular mechanisms of biliary-driven liver regeneration. Moreover, these methods can be applied to chemical screens to identify small molecules that augment or suppress liver regeneration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping